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. 2021 Dec 17;31(1):131-138.
doi: 10.1007/s10068-021-01011-w. eCollection 2022 Jan.

Physicochemical stability and virucidal effect of diluted, slightly acidic electrolyzed water against human norovirus

Hae-Won Lee  1 , Boyeon Park  1 , So-Ra Yoon  1 , Ji-Su Yang  1 , Ji-Hyoung Ha  1
Affiliations

Physicochemical stability and virucidal effect of diluted, slightly acidic electrolyzed water against human norovirus

Hae-Won Lee et al. Food Sci Biotechnol. .

Abstract

This study aimed to evaluate the virucidal effect and potential use as a disinfectant of undiluted and diluted slightly acidic electrolyzed water (SAEW) on human noroviruses (HuNoVs) using the in vitro suspension test and in food test. The oxidization reduction potential of SAEW gradually decreased with the increase in distilled water volume. Moreover, as the volume of distilled water and the dilution ratio increased, the available chlorine concentration of the samples significantly decreased from 31.2 ± 0.63 (SAEW) to 1.3 ± 0.21 (1:10 dilution of SAEW solution) (p < 0.05). Undiluted SAEW presented the lowest pH (5.56 ± 0.02) and as SAEW was diluted in distilled water, the pH of the sample increased. Considering the standard reduction values of pathogenic virus (> 4.00 log reduction), the reduction value of HuNoVs in cabbage samples was 4.65 (GI.6) and 4.28 (GII.4) log. These results suggest the potential application of SAEW for inactivating HuNoVs in the food industry.

Supplementary information: The online version contains supplementary material available at 10.1007/s10068-021-01011-w.

Keywords: Cabbage; Disinfection; Human norovirus; Physicochemical; Slightly acidic electrolyzed water.

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Conflict of interest statement

Conflict of interestThe authors have no competing interest to declare.

Figures

Fig. 1
Fig. 1
Changes in the physicochemical properties of undiluted and diluted SAEW and HuNoV GI.6 reduction values by disinfectant condition used in the suspension test. A-G indicate a significant (p < 0.05) difference within the log reduction values. SAEW, slightly acidic electrolyzed water; HuNoV, human norovirus
Fig. 2
Fig. 2
Changes in the physicochemical properties of undiluted and diluted SAEW and HuNoV GII.4 reduction values by disinfectant condition used in the suspension test. SAEW, slightly acidic electrolyzed water; HuNoV, human norovirus
Fig. 3
Fig. 3
Correlation coefficient matrix of HuNoVs and physicochemical properties. A-1 and A-2 show the suspension test results and B-1 and B-2 the food test results. Positive coefficients represented by blue circles indicate a direct relationship between variables in the matrix; negative coefficients shown by using red circles reflect an inverse relationship. HuNoVs, human noroviruses
See this image and copyright information in PMC

References

    1. Al-Haq MI, Sugiyama J, Isobe S. Applications of electrolyzed water in agriculture and food industries. Food Sci. Technol. Res. . 2005;11:135–150. doi: 10.3136/fstr.11.135. - DOI
    1. Cui X, Shang Y, Shi Z, Xin H, Cao W. Physicochemical properties and bactericidal efficiency of neutral and acidic electrolyzed water under different storage conditions. J Food En. 2009;9:582–586. doi: 10.1016/j.jfoodeng.2008.10.006. - DOI
    1. Diez-Valcarce M, Lopez MR, Lopez B, Morales O, Sagastume M, Cadena L, Kaydos-Daniels S, Jarquin C, McCracken JP, Bryan JP, Vinjé J. Prevalence and genetic diversity of viral gastroenteritis viruses in children younger than 5 years of age in Guatemala, 2014–2015. J Clin Vir. 2019;114:6–11. doi: 10.1016/j.jcv.2019.03.006. - DOI - PMC - PubMed
    1. European Committee for Standardization (CEN). EN 1276: Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas - Test method and requirements (phase 2, step 1). Brussels, Belgium (2019)
    1. Griffin SM, Brinkman NE, Hedrick EJ, Rhodes ER, Fout GS. Comparison of nucleic acid extraction and reverse transcription-qPCR approaches for detection of GI and GII noroviruses in drinking water. Journal of Virological Methods. 2014;199:76–85. doi: 10.1016/j.jviromet.2014.01.005. - DOI - PubMed

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