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. 2022 Jan 21;17(1):e0259668.
doi: 10.1371/journal.pone.0259668. eCollection 2022.

72-Hour transport recovery of antimicrobial resistant Neisseria gonorrhoeae isolates using the InTray® GC method

Keely S Paris  1 , Brandon Font  2 , Sanjay R Mehta  3 , Irvin Huerta  2 , Claire C Bristow  3
Affiliations

72-Hour transport recovery of antimicrobial resistant Neisseria gonorrhoeae isolates using the InTray® GC method

Keely S Paris et al. PLoS One. .

Abstract

Recovery of Neisseria gonorrhoeae isolates exposed to a range of transport times and temperatures was quantitatively assessed for two transport devices, BioMed Diagnostics' InTray GC® and Copan Diagnostics' Liquid Amies Elution Swab (ESwab®) Collection and Transport System. Respective devices were inoculated with N. gonorrhoeae, exposed to simulated transport conditions and spread-plated from serial dilutions in duplicates onto chocolate agar in order to count CFU (colony-forming units) in the range of 25-250. Baseline CFU/mL averages of time-zero transport for each device was compared to either 24 hour (Eswab) or 72 hour (InTray GC) CFU/mL average to assess recovery of six clinical isolates of N. gonorrhoeae, and differences showing no greater than a 3 log10 (± 10%) decline between comparative time points qualified as acceptable. Our findings suggest that the InTray GC system has the potential to transport clinical isolates for ≤72 hours with acceptable N. gonorrhoeae recovery.

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Conflict of interest statement

The authors have read the journal’s policy and have the following competing interests: BF and IH are employees of Biomed Diagnostics Inc., which provided funding for all study procedures. InTray® GC is a registered trademark of BioMed Diagnostics Inc., which also owns a patent for the InTray® GC platform. This does not alter our adherence to PLOS ONE policies on sharing data and materials.

Figures

Fig 1
Fig 1. Methods flowchart for the InTray GC and ESwab transport recovery of Neisseria gonorrhoeae isolates.
Fig 2
Fig 2. Time in hours versus Log10 CFU/mL for the InTray GC for all six strains.
CFU; colony forming units.
Fig 3
Fig 3. Time in hours versus Log10 CFU/mL for the Copan ESwab for all six strains.
CFU; colony forming units.
See this image and copyright information in PMC

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