Association between household air pollution and nasopharyngeal pneumococcal carriage in Malawian infants (MSCAPE): a nested, prospective, observational study

Abstract

Background: Household air pollution from solid fuels increases the risk of childhood pneumonia. Nasopharyngeal carriage of Streptococcus pneumoniae is a necessary step in the development of pneumococcal pneumonia. We aimed to assess the association between exposure to household air pollution and the prevalence and density of S pneumoniae carriage among children.

Methods: The Malawi Streptococcus pneumoniae Carriage and Air Pollution Exposure study was a nested, prospective, observational study of children participating in the cluster randomised controlled Cooking and Pneumonia Study (CAPS) in the Karonga Health and Demographic Surveillance System (HDSS) area in northern Malawi. CAPS compared the effects of a cleaner burning biomass-fuelled cookstove (intervention group) with traditional open-fire cooking (control group) on the incidence of pneumonia in children. Eligible children aged 6 weeks or 6 months (those recruited a 6 weeks were also followed up at age 6 months) were identified by the Karonga HDSS centre. Nasopharyngeal swabs were taken to detect S pneumoniae, and infant exposure to particulate matter with a diameter of ≤2·5 μm (PM_2·5) exposure was assessed by use of a MicroPEM device. The primary outcome was the prevalence of nasopharyngeal S pneumoniae carriage in all children aged 6 months, assessed in all children with valid data on PM_2·5. The effects of the intervention stoves (intention-to-treat analysis) and PM_2·5 (adjusted exposure-response analysis) on the prevalence of S pneumoniae carriage were also assessed in the study children.

Findings: Between Nov 15, 2015, and Nov 2, 2017, 485 children were recruited (240 from the intervention group and 245 from the control group). Of all 450 children with available data at age 6 months, 387 (86% [95% CI 82-89]) were positive for S pneumoniae. Geometric mean PM_2·5 exposure was 60·3 μg/m³ (95% CI 55·8-65·3) in S pneumoniae-positive children and 47·0 μg/m³ (38·3-57·7) in S pneumoniae-negative children (p=0·044). In the intention-to-treat analysis, a non-significant increase in the risk of S pneumoniae carriage was observed in intervention group children compared with control group children (odds ratio 1·36 [95% CI 0·95-1·94]; p=0·093). In the exposure-response analysis, a significant association between PM_2·5 exposure and S pneumoniae carriage was observed; a one unit increase in decile of PM_2·5 was found to significantly increase the risk of S pneumoniae carriage by 10% (1·10 [1·01-1·20]; p=0·035), after adjustment for age, sex, 13-valent pneumococcal conjugate vaccination status, season, current use of antibiotics, and MicroPEM run-time.

Interpretation: Despite the absence of effect from the intervention cookstove, household air pollution exposure was significantly associated with the prevalence of nasopharyngeal S pneumoniae carriage. These results provide empirical evidence for the potential mechanistic association between exposure to household air pollution and childhood pneumonia.

Funding: Bill & Melinda Gates Foundation.

Figure 1

Recruitment of the MSCAPE study children from within the CAPS CAPS=Cooking And Pneumonia Study. MSCAPE=Malawi Streptococcus pneumoniae Carriage and Air Pollution Exposure. PM=particulate matter.

Figure 2

Box plot showing the distributions of average PM_2·5 exposure by nasopharyngeal Streptococcus pneumoniae carriage status in children aged 6 weeks and 6 months Results are reported on a log scale. The thick black lines inside the boxes indicate the median values, the box representes the IQR, the whisker limits span 1·5 × the IQR, and the filled circles are outliers. PM=particulate matter.