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. 1987 Jan-Feb;4(1):9-13.
doi: 10.1016/0735-0651(87)90007-0.

Analysis of closely related genes by the use of synthetic oligonucleotide probes labeled to a high specific activity

L Hellman  1 , U Pettersson
Affiliations

Analysis of closely related genes by the use of synthetic oligonucleotide probes labeled to a high specific activity

L Hellman et al. Gene Anal Tech. 1987 Jan-Feb.

Abstract

A method for labeling synthetic oligonucleotide probes to high specific activity is described. The method utilizes two partly complementary oligonucleotides that are labeled by a fill-in reaction using the Klenow fragment of DNA polymerase I and four alpha 32P-nucleoside triphosphates. Such probes can, in combination with Southern blot analysis, be used for routine analysis of individual genes in multigene families.

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