A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane

Abstract

Amniotic membrane (AM) has been utilized as a wound dressing extensively. Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM. This construct was composed of H₂O₂-loaded polylactic acid (PLA) microparticles embedded within a chitosan/β-glycerophosphate (β-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM. The microparticles had a diameter of 4.48 ± 1.8 μm, an encapsulation efficiency of 44.172 ± 4.49%, and generated oxygen for at least 7 days. The hybrid construct was formed at 32.4 ± 2 °C, had a porous structure (84.69 ± 8.34%) with a pore size of 46.72 ± 26.21 μm. The hydrogel/dAM extract was non-toxic after 7 days based on our MTT results, and the final composite supported cell growth and adhesion. This sample had the most negligible blood cell adhesion with less than 5% hemolysis. Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.

Keywords: 2,2-Diphenyl-1-picrylhydrazyl; Amniotic membrane; Catalase; Chitosan; Chitosan temperature-sensitive hydrogel; H(2)O(2): hydrogen peroxide; HBOT: hyperbaric oxygen therapy; Hydrogen peroxide; MTT: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; PLA microparticles; Poly L-lactic acid; Poly-vinyl alcohol; Poly-vinyl pyrrolidone; Wound dressing; β-Glycerophosphate.