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. 1986 Jun;2(2):196-200.

Effect of nutrient concentration in culturing three isolates of the mosquito fungal pathogen, Lagenidium giganteum (Oomycetes: Lagenidiales), on sunflower seed extract

D R Guzman  1 , R C Axtell
Affiliations
  • PMID: 3507488

Effect of nutrient concentration in culturing three isolates of the mosquito fungal pathogen, Lagenidium giganteum (Oomycetes: Lagenidiales), on sunflower seed extract

D R Guzman et al. J Am Mosq Control Assoc. 1986 Jun.

Abstract

The requirements for soluble protein concentration in sunflower seed extract (SFE) cultures were similar for 3 isolates of Lagenidium giganteum from North Carolina, Louisiana and California. Sunflower seed extract agar containing soluble protein in the range of 1.0 to 4.0 mg/ml was satisfactory for high levels of zoospore production. It is recommended that the protein concentration be 2.0 mg/ml for both SFE agar and liquid SFE culturing media, and the protein assay be with the Bradford method using bovine serum albumin as the standard.

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