Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Multicenter Study
. 2022 Jan 24;12(1):1237.
doi: 10.1038/s41598-022-05315-7.

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

David Rodriguez-Temporal  1 , Fernando Alcaide  2 , Ivana Mareković  3 , James Anthony O'Connor  4 , Rebecca Gorton  5 , Jakko van Ingen  6 , An Van den Bossche  7 , Genevieve Héry-Arnaud  8 , Clémence Beauruelle  8 , Dorothea Orth-Höller  9 , Juan-José Palacios-Gutiérrez  10 , Griselda Tudó  11 , Germán Bou  12 , Pieter-Jan Ceyssens  7 , Montserrat Garrigó  13 , Julià González-Martin  11 , Gilbert Greub  14 , Jaroslav Hrabak  15 , André Ingebretsen  16 , Maria Concepción Mediavilla-Gradolph  17 , Marina Oviaño  12 , Begoña Palop  17 , Arthur B Pranada  18 , Lidia Quiroga  19 , Maria Jesús Ruiz-Serrano  19 , Belén Rodríguez-Sánchez  20
Affiliations
Multicenter Study

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

David Rodriguez-Temporal et al. Sci Rep. .

Abstract

The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Distribution of isolates by centres and MALDI-TOF MS log(score).
Figure 2
Figure 2
Log(score) obtained in all centres for slow growing species.
Figure 3
Figure 3
Log(score) obtained in all centres for rapid growing species.
See this image and copyright information in PMC

References

    1. Porvaznik I, Solovic I, Mokry J. Non-tuberculous mycobacteria: Classification, diagnostics, and therapy. Adv. Exp. Med. Biol. 2017;944:19–25. doi: 10.1007/5584_2016_45. - DOI - PubMed
    1. van Ingen J. Microbiological diagnosis of nontuberculous mycobacterial pulmonary disease. Clin. Chest Med. 2015;36:43–54. doi: 10.1016/j.ccm.2014.11.005. - DOI - PubMed
    1. Griffith DE, et al. An official ATS/IDSA statement: Diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am. J. Respir. Crit. Care Med. 2007;175:367–416. doi: 10.1164/rccm.200604-571ST. - DOI - PubMed
    1. Haworth CS, et al. British Thoracic Society guidelines for the management of non-tuberculous mycobacterial pulmonary disease (NTM-PD) Thorax. 2017;72:ii1–ii64. doi: 10.1136/thoraxjnl-2017-210927. - DOI - PubMed
    1. Rodriguez-Sanchez B, et al. Evaluation of MALDI biotyper mycobacteria library v3.0 for identification of nontuberculous mycobacteria. J. Clin. Microbiol. 2016;54:1144–1147. doi: 10.1128/JCM.02760-15. - DOI - PMC - PubMed

Publication types

MeSH terms