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Review
. 2022 Feb 28;50(1):375-386.
doi: 10.1042/BST20210246.

Mechanism of action of nucleotide excision repair machinery

Areetha D'Souza  1 , Alexandra M Blee  1 , Walter J Chazin  1
Affiliations
Review

Mechanism of action of nucleotide excision repair machinery

Areetha D'Souza et al. Biochem Soc Trans. .

Abstract

Nucleotide excision repair (NER) is a versatile DNA repair pathway essential for the removal of a broad spectrum of structurally diverse DNA lesions arising from a variety of sources, including UV irradiation and environmental toxins. Although the core factors and basic stages involved in NER have been identified, the mechanisms of the NER machinery are not well understood. This review summarizes our current understanding of the mechanisms and order of assembly in the core global genome (GG-NER) pathway.

Keywords: DNA synthesis and repair; nucleotide excision repair; structural biology.

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Conflict of interest statement

Competing Interests

The authors declare that there are no competing interests associated with the manuscript.

Figures

Figure 1.
Figure 1.. The trajectory of dynamic assembly of the core GG-NER machinery.
Four steps are identified: (A) damage recognition, (B) damage verification, (C) pre-incision, and (D) dual incision.
Figure 2.
Figure 2.. Cryo-EM structures of TFIIH complexe.
(A) Yeast TFIIH and XPC-HR23B-Cen2. (B) Human TFIIH and XPA.
See this image and copyright information in PMC

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