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Review
. 2022:2441:201-221.
doi: 10.1007/978-1-0716-2059-5_16.

Quantitative Methods to Assess Adipose Vasculature

Martina Rudnicki  1 , Alexandra Pislaru  2 , Tara L Haas  3
Affiliations
Review

Quantitative Methods to Assess Adipose Vasculature

Martina Rudnicki et al. Methods Mol Biol. 2022.

Abstract

Adipose tissue depots are invested with an extensive capillary network that is closely associated with maintenance of adipose functions and enables healthy tissue expansion. The capillary network displays a high level of plasticity, demonstrating either growth (angiogenesis) or regression (rarefaction) under various physiological/pathological conditions, which has significant consequences for cardiometabolic health. Thus, the visualization and quantification of adipose vascular networks is an important aspect of studying factors that regulate adipose tissue health. This chapter provides an overview of several methods to quantify adipose vascularization. In-depth protocols are provided for the visualization of vascular structures by staining and imaging of whole-mount adipose tissues or paraffin-embedded adipose tissue sections, together with the quantitative analysis of vascularization from these images.

Keywords: Adipose imaging; Angiogenesis; Capillary density; Capillary morphology; Endothelial markers; Whole-mount adipose.

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