A Starter Guide to Immunofluorescence Testing in Dermatology

Abstract

Immunodermatology laboratory testing, including direct immunofluorescence (DIF), indirect immunofluorescence (IIF), and enzyme-linked immunosorbent assay (ELISA) are powerful tools that can aide dermatologists when diagnosing autoimmune blistering diseases. Understanding these tests is important to ensure appropriate use and optimum results. This article is intended to serve as a helpful primer for immunofluorescence testing in dermatology, with an overview of the tests available as well as pragmatic tips for optimal biopsy sites and specimen transport.