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. 2022 Feb 1;8(1):8.
doi: 10.1038/s41421-021-00358-y.

DdCBE-mediated mitochondrial base editing in human 3PN embryos

Xiaoxu Chen #  1 , Dong Liang #  2 , Jiayin Guo #  1   3 , Junqiang Zhang #  4 , Haifeng Sun #  1 , Xiaolan Zhang  4 , Jiachuan Jin  1 , Yichen Dai  1 , Qinmin Bao  2 , Xuezhen Qian  1 , Lei Tan  1 , Ping Hu  2 , Xiufeng Ling  5 , Bin Shen  6   7   8   9 , Zhengfeng Xu  10
Affiliations

DdCBE-mediated mitochondrial base editing in human 3PN embryos

Xiaoxu Chen et al. Cell Discov. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1. DdCBE-mediated mtDNA base editing in human 3PN embryos.
a Sequence chromatograms of maternal peripheral blood mononuclear cells (PBMCs) and 3PN embryos injected with G3733-DdCBE mRNAs. #20-UC and #29-HiFi embryos are derived from the same donor. The target site is indicated by the red arrow. b Sequence chromatograms of maternal PBMCs and 3PN embryos injected with G8363-DdCBE mRNAs. #29-UC and #27-HiFi embryos are derived from different donors. c, d Frequencies of C ∙ G-to-T ∙ A conversion within the spacing region of G3733A (c) and G8363A (d) embryos. Each dot represents an embryo. e Frequencies of off-target C ∙ G-to-T ∙ A conversions along the whole mtDNA of maternal PBMC and G3733A embryos. The frequency of on-target editing is labeled behind the embryo number. The four 3PN embryos are from the same donor. f Average frequency of mtDNA-wide C ∙ G-to-T ∙ A conversions for each DdCBE pair. Data are presented as means ± SD (n = 4 for unedited controls and G3733A embryos, n = 3 for G8363A embryos). Significance was calculated with unpaired two-tailed Student’s t-test (* P < 0.05, ** P < 0.01).
See this image and copyright information in PMC

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