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. 2022 Jan 31;12(1):9.
doi: 10.1186/s13568-022-01351-8.

Antibacterial effect on microscale rough surface formed by fine particle bombarding

Tomoko Nishitani  1 , Kyosuke Masuda  2 , Soma Mimura  2 , Takahiko Hirokawa  3 , Hitoshi Ishiguro  3 , Masao Kumagai  4 , Takeshi Ito  2
Affiliations

Antibacterial effect on microscale rough surface formed by fine particle bombarding

Tomoko Nishitani et al. AMB Express. .

Abstract

Fine particle bombarding (FPB) is typically utilized to modify metal surfaces by bombarding them with fine particles at high-speed. The diameters of the particles range from several to tens of micrometers. FPB forms fine microscale concavities and convexities on a surface. As FPB-treated surfaces are widely used in the food industry, the influence of bacteria on their surface must be considered. In this study, we examined the antibacterial activity of microscale rough surfaces formed by FPB. We applied FPB to a stainless-steel surface and evaluated the antibacterial effect of FPB-treated surfaces based on JIS Z 2801 (a modified test method from ISO 22196:2007). Our results indicated that the FPB-treated surfaces (FPB-1 (avg. pitch: 0.72 µm) and FPB-2 (avg. pitch: 3.56 µm)) exhibited antibacterial activity both against Escherichia coli and Staphylococcus aureus.

Keywords: Antibacterial effect; Fine particle bombarding; Microscale roughness; Surface shape.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
3D images of control and FPB surfaces. a Control, b FPB-1, c FPB-2, d FPB-3, and e FPB-4
Fig. 2
Fig. 2
Cross-sectional profile curves of control and FPB-treated surfaces. a Control, b FPB-1, c FPB-2, d FPB-3, and e FPB-4.
Fig. 3
Fig. 3
Images for evaluating the WCA of control and FPB-treated surfaces. a Control, WCA = 98.5°, b FPB-1, WCA = 65.5°, c FPB-2, WCA = 85.4°, d FPB-3, WCA = 90.0°, and e FPB-4, WCA = 92.3°
Fig. 4
Fig. 4
Relationship between the antibacterial activity score and roughness pitch of FPB-treated surfaces
Fig. 5
Fig. 5
Relationship between the viable bacterial count and WCA on the control and FPB-treated surfaces
Fig. 6
Fig. 6
Variation in active cell ratio of FPB-2 with time, obtained from the results of live/dead assay test. Number of samples (N) was 3
Fig. 7
Fig. 7
SEM image of the FPB-2 treated surface 60 min after the adhesion of E. coli. Arrows indicate E. coli
See this image and copyright information in PMC

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