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. 2022 Apr;51(4):405-412.
doi: 10.1111/jop.13282. Epub 2022 Mar 7.

Quantitative proteomic study reveals differential expression of matricellular proteins between fibrous dysplasia and cemento-ossifying fibroma pathogenesis

Filipe Fideles Duarte-Andrade  1 , Thais Dos Santos Fontes Pereira  1 , Jéssica Gardone Vitório  1 , Marina Gonçalves Diniz  2 , Larissa Stefhanne Damasceno Amorim  1 , Arkadiusz Nawrocki  3 , Liza Figueiredo Felicori  4 , Luiz De Marco  5 , Carolina Cavaliéri Gomes  2 , Martin R Larsen  3 , Marcella Nunes Melo-Braga  4 , Ricardo Santiago Gomez  1
Affiliations

Quantitative proteomic study reveals differential expression of matricellular proteins between fibrous dysplasia and cemento-ossifying fibroma pathogenesis

Filipe Fideles Duarte-Andrade et al. J Oral Pathol Med. 2022 Apr.

Abstract

Background: Fibrous dysplasia (FD) and cemento-ossifying fibroma (COF) are the most common gnathic fibro-osseous lesions. These diseases exhibit remarkable overlap of several clinicopathological aspects, and differential diagnosis depends on the combination of histopathological, radiographic, and clinical aspects. Their molecular landscape remains poorly characterized, and herein, we assessed their proteomic and phosphoproteomic profiles.

Methods: The quantitative differences in protein profile of FD and COF were assessed by proteomic and phosphoproteomic analyses of formalin-fixed paraffin-embedded tissue samples. Pathway enrichment analyses with differentially regulated proteins were performed.

Results: FD and COF exhibited differential regulation of pathways related to extracellular matrix organization, cell adhesion, and platelet and erythrocytes activities. Additionally, these lesions demonstrated distinct abundance of proteins involved in osteoblastic differentiation and tumorigenesis and differential abundance of phosphorylation of Ser61 of Yes-associated protein 1 (YAP1).

Conclusions: In summary, despite the morphological similarity between these diseases, our results demonstrated that COF and DF present numerous quantitative differences in their proteomic profiles. These findings suggest that these fibro-osseous lesions trigger distinct molecular mechanisms during their pathogenesis. Moreover, some proteins identified in our analysis could serve as potential biomarkers for differential diagnosis of these diseases after further validation.

Keywords: bone tumors; fibro-osseous lesions; fibrous dysplasia; ossifying fibroma; proteomics.

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References

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