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. 2022 Apr;102(4):115638.
doi: 10.1016/j.diagmicrobio.2022.115638. Epub 2022 Jan 16.

IgG anti-spike antibody levels in healthcare workers with and without prior COVID-19 up to 3 months after BNT162b2 vaccination

Jan Van Elslande  1 , Matthias Weemaes  1 , Lode Godderis  2 , Gijs Van Pottelbergh  3 , Xavier Bossuyt  4 , Pieter Vermeersch  5
Affiliations

IgG anti-spike antibody levels in healthcare workers with and without prior COVID-19 up to 3 months after BNT162b2 vaccination

Jan Van Elslande et al. Diagn Microbiol Infect Dis. 2022 Apr.

Abstract

We report IgG anti-spike levels up to 3 months after vaccination with 2 doses of BNT162b2 mRNA vaccine in healthcare workers (HCW). The antibody response was significantly stronger in previously infected vaccinated HCW compared to uninfected HCW, and stronger after vaccination compared to (mostly) mild natural infection.

Keywords: COVID-19; COVID-19 testing; IgG; Immunoassay; SARS-CoV-2; Spike; Vaccination.

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Figures

Fig 1
Fig. 1
IgG anti-spike antibody response after vaccination and after natural infection. (A) Anti-S antibody titers in vaccinated HCW with (pink) and without (light green) a prior SARS-CoV-2 infection (time points indicating time after first vaccine dose), hospitalized COVID-19 patients (blue) and non-vaccinated HCW after natural infection (red) (time points indicating time after first positive SARS-Cov-2 PCR). The boxplots indicate the median, 25th and 75th percentile. (B) dynamic evolution of anti-S antibody levels. The manufacturer's cut-off for positivity of 50 AU/mL (1.7 on the log10 scale) and the cut-off for high probability of neutralizing antibody titer at 4160 AU/mL (3.6 on the log10 scale) are depicted as long dashed and short dashed horizontal black lines, respectively. *P < 0.001 compared to vaccinated HCW with prior COVID-19 at the same time point, †P < 0.001 vs all groups at 3 months. (Color version of figure is available online.)
See this image and copyright information in PMC

References

    1. Bayart JL, Douxfils J, Gillot C, David C, Mullier F, Elsen M, et al. Waning of IgG, total and neutralizing antibodies 6 months post-vaccination with BNT162b2 in healthcare workers. Vaccines. 2021;9:1092. doi: 10.3390/vaccines9101092. - DOI - PMC - PubMed
    1. Dagan N, Barda N, Kepten E, Miron O, Percheck S, Katz M, et al. BNT162b2 mRNA Covid-19 Vaccine in a Nationwide Mass Vaccination Setting. N Engl J Med. 2021;384:1412–1423. doi: 10.1056/NEJMoa2101765. - DOI - PMC - PubMed
    1. Ebinger JE, Fert-Bober J, Printsev I, Wu M, Sun N, Prostko JC, et al. Antibody responses to the BNT162b2 mRNA vaccine in individuals previously infected with SARS-CoV-2. Nat Med. 2021;27:981–984. doi: 10.1038/s41591-021-01325-6. - DOI - PMC - PubMed
    1. Favresse J, Bayart J-L, Mullier F, Dogné J-M, Closset M, Douxfils J. Early antibody response in healthcare professionals after two doses of SARS-CoV-2 mRNA vaccine (BNT162b2) Clin Microbiol Infect. 2021;27:1351.e5–1351.e7. doi: 10.1016/j.cmi.2021.05.004. - DOI - PMC - PubMed
    1. Khoury DS, Cromer D, Reynaldi A, Schlub TE, Wheatley AK, Juno JA, et al. Neutralizing antibody levels are highly predictive of immune protection from symptomatic SARS-CoV-2 infection. Nat Med. 2021;27:1205–1211. doi: 10.1038/s41591-021-01377-8. - DOI - PubMed