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. 2022 Apr 11;61(16):e202114504.
doi: 10.1002/anie.202114504. Epub 2022 Feb 19.

A Polymeric Nanobeacon for Monitoring the Fluctuation of Hydrogen Polysulfides during Fertilization and Embryonic Development

Yibo Zhou  1 , Zhengxuan Gu  1 , Changhui Liu  2 , Sheng Yang  3 , Xiaofei Ma  1 , Qiaoshu Chen  4 , Yanli Lei  1 , Ke Quan  1 , Juewen Liu  4 , Zhihe Qing  1 , Ronghua Yang  3
Affiliations

A Polymeric Nanobeacon for Monitoring the Fluctuation of Hydrogen Polysulfides during Fertilization and Embryonic Development

Yibo Zhou et al. Angew Chem Int Ed Engl. .

Abstract

Fertilization and early embryonic development as the beginning of a new life are key biological events. Hydrogen polysulfide (H2 Sn ) plays important roles during physiological regulation, such as antioxidation-protection. However, no report has studied in situ H2 Sn fluctuation during early embryonic development because of the low abundance of H2 Sn and inadequate sensitivity of probes. We herein construct a polymeric nanobeacon from a H2 Sn -responsive polymer and fluorophores, which is capable of detecting H2 Sn selectively and of signal amplification. Taking the zebrafish as a model, the polymeric nanobeacon revealed that the H2 Sn level was significantly elevated after fertilization due to the activation of cell multiplication, suppressed partially during embryonic development, and finally kept steady up to zebrafish emergence. This strategy is generally accessible for biomarkers by altering the responsive unit and significant for facilitating biological analysis during life development.

Keywords: Embryonic Development; Fluorescence Imaging; Hydrogen Polysulfides; Polymeric Nanobeacon; Signal Amplification.

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