doi: 10.1016/s0006-291x(86)80482-x.
p60c-src activity detected in the chromaffin granule membrane
- PMID: 3511908
- DOI: 10.1016/s0006-291x(86)80482-x
Item in Clipboard
p60c-src activity detected in the chromaffin granule membrane
Biochem Biophys Res Commun.
.
Abstract
Using monoclonal antibodies specific for p60c-src we have detected high levels of this kinase in adrenal medullary chromaffin tissue and in highly purified chromaffin granule (secretory vesicle) membranes. An immune complex kinase assay was applied to fractions of adrenal medullary tissue resolved on sucrose density gradients. Thirty-seven per cent of the total tissue p60c-src activity was found in association with chromaffin granule or granule membrane markers. Localization of a significant fraction of total cellular p60c-src activity to this secretory vesicle membrane suggests that the kinase may function in the regulation of neurotransmitter release.
Similar articles
-
Secretory vesicle - cytosol interactions in exocytosis: isolation by Ca2+-dependent affinity chromatography of proteins that bind to the chromaffin granule membrane.Biochem Biophys Res Commun. 1981 Dec 31;103(4):1395-400. doi: 10.1016/0006-291x(81)90278-3. Biochem Biophys Res Commun. 1981. PMID: 7332599 No abstract available.
-
Chromaffin granule membrane-F-actin interactions and spectrin-like protein of subcellular organelles: a possible relationship.J Neurochem. 1984 Jun;42(6):1558-69. doi: 10.1111/j.1471-4159.1984.tb12742.x. J Neurochem. 1984. PMID: 6374036
-
Protein phosphorylation and the exocytosis-like interaction between isolated adrenal medullary plasma membranes and chromaffin granules.Biochem Biophys Res Commun. 1983 Jan 14;110(1):55-60. doi: 10.1016/0006-291x(83)91259-7. Biochem Biophys Res Commun. 1983. PMID: 6687680
-
Role of calcium in secretion and synthesis in bovine adrenal chromaffin cells.Adv Exp Med Biol. 1990;269:93-7. doi: 10.1007/978-1-4684-5754-4_14. Adv Exp Med Biol. 1990. PMID: 2191566 Review. No abstract available.
-
Molecular pharmacology of the monoamine transporter of the chromaffin granule membrane.Ann N Y Acad Sci. 1987;493:194-206. doi: 10.1111/j.1749-6632.1987.tb27201.x. Ann N Y Acad Sci. 1987. PMID: 3296907 Review. No abstract available.
Cited by
-
Translocation of pp60c-src to the cytoskeleton during platelet aggregation.EMBO J. 1992 Mar;11(3):855-61. doi: 10.1002/j.1460-2075.1992.tb05123.x. EMBO J. 1992. PMID: 1372252 Free PMC article.
-
Src kinases regulate de novo actin polymerization during exocytosis in neuroendocrine chromaffin cells.PLoS One. 2014 Jun 5;9(6):e99001. doi: 10.1371/journal.pone.0099001. eCollection 2014. PLoS One. 2014. PMID: 24901433 Free PMC article.
-
Specific association of the proto-oncogene product pp60c-src with an intracellular organelle, the PC12 synaptic vesicle.J Cell Biol. 1992 Jun;117(5):1077-84. doi: 10.1083/jcb.117.5.1077. J Cell Biol. 1992. PMID: 1374414 Free PMC article.
-
Biological and biochemical properties of the c-src+ gene product overexpressed in chicken embryo fibroblasts.Mol Cell Biol. 1989 Aug;9(8):3332-41. doi: 10.1128/mcb.9.8.3332-3341.1989. Mol Cell Biol. 1989. PMID: 2477684 Free PMC article.
-
A 42-kD tyrosine kinase substrate linked to chromaffin cell secretion exhibits an associated MAP kinase activity and is highly related to a 42-kD mitogen-stimulated protein in fibroblasts.J Cell Biol. 1990 Mar;110(3):731-42. doi: 10.1083/jcb.110.3.731. J Cell Biol. 1990. PMID: 1689732 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
