Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress

Paweł Krzemień¹, Sławomir Kasperczyk², Maciej Banach³, Aleksandra Kasperczyk², Michał Dobrakowski², Tomasz Tomasik⁴, Adam Windak⁴, Mirosław Mastej⁵, Alberico Catapano⁶, Kausik K Ray⁷, Dimitri P Mikhailidis⁸, Peter P Toth^{9

10}, George Howard¹¹, Gregory Yh Lip^{12

13}, Maciej Tomaszewski¹⁴, Fadi J Charchar¹⁵, Naveed Sattar¹⁶, Bryan Williams¹⁷, Thomas M MacDonald¹⁸, Peter E Penson¹⁹, Jacek J Jóźwiak²⁰

Affiliations

¹ Euroimmun Polska Sp. z o.o., Wroclaw, Poland.
² Department of Biochemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland.
³ Department of Hypertension, Medical University of Lodz, Łódź, Poland.
⁴ Department of Family Medicine, Jagiellonian University Medical College, Krakow, Poland.
⁵ Mastej Medical Center, Jasło, Poland.
⁶ Department of Pharmacological Sciences, University of Milano and Multimedica IRCCS, Milano, Italy.
⁷ Department of Primary Care and Public Health, Imperial Centre for Cardiovascular Disease Prevention, Imperial College, Kensington, London, UK.
⁸ Department of Clinical Biochemistry, Royal Free Hospital, University College London, London, UK.
⁹ Cicarrone Center for the Prevention of Cardiovascular Disease, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
¹⁰ CGH Medical Center, Sterling, IL, USA.
¹¹ Department of Biostatistics, School of Public Health of Alabama at Birmingham, Birmingham, AL, USA.
¹² Liverpool Centre for Cardiovascular Science, University of Liverpool and Liverpool Heart & Chest Hospital, Liverpool, UK.
¹³ Department of Clinical Medicine, Aalborg University, Aalborg, Denmark.
¹⁴ Division of Cardiovascular Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
¹⁵ School of Health and Life Sciences, Federation University Australia, Ballarat, VIC, Australia.
¹⁶ Institute of Cardiovascular and Medical Science, University of Glasgow, Glasgow, UK.
¹⁷ NIHR University College London Biomedical Research Centre, University College London and University College London Hospitals NHS Foundation Trust, London, UK.
¹⁸ MEMO Research, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK.
¹⁹ Liverpool Centre for Cardiovascular Science, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, UK.
²⁰ Department of Family Medicine and Public Health, Faculty of Medicine, University of Opole, Opole, Poland.

PMID: 35125863
PMCID: PMC8808033
DOI: 10.1177/11772719211066791

Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress

Paweł Krzemień et al. Biomark Insights. 2022.

. 2022 Jan 30:17:11772719211066791.

doi: 10.1177/11772719211066791. eCollection 2022.

Authors

Affiliations

¹ Euroimmun Polska Sp. z o.o., Wroclaw, Poland.
² Department of Biochemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland.
³ Department of Hypertension, Medical University of Lodz, Łódź, Poland.
⁴ Department of Family Medicine, Jagiellonian University Medical College, Krakow, Poland.
⁵ Mastej Medical Center, Jasło, Poland.
⁶ Department of Pharmacological Sciences, University of Milano and Multimedica IRCCS, Milano, Italy.
⁷ Department of Primary Care and Public Health, Imperial Centre for Cardiovascular Disease Prevention, Imperial College, Kensington, London, UK.
⁸ Department of Clinical Biochemistry, Royal Free Hospital, University College London, London, UK.
⁹ Cicarrone Center for the Prevention of Cardiovascular Disease, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
¹⁰ CGH Medical Center, Sterling, IL, USA.
¹¹ Department of Biostatistics, School of Public Health of Alabama at Birmingham, Birmingham, AL, USA.
¹² Liverpool Centre for Cardiovascular Science, University of Liverpool and Liverpool Heart & Chest Hospital, Liverpool, UK.
¹³ Department of Clinical Medicine, Aalborg University, Aalborg, Denmark.
¹⁴ Division of Cardiovascular Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
¹⁵ School of Health and Life Sciences, Federation University Australia, Ballarat, VIC, Australia.
¹⁶ Institute of Cardiovascular and Medical Science, University of Glasgow, Glasgow, UK.
¹⁷ NIHR University College London Biomedical Research Centre, University College London and University College London Hospitals NHS Foundation Trust, London, UK.
¹⁸ MEMO Research, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK.
¹⁹ Liverpool Centre for Cardiovascular Science, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, UK.
²⁰ Department of Family Medicine and Public Health, Faculty of Medicine, University of Opole, Opole, Poland.

PMID: 35125863
PMCID: PMC8808033
DOI: 10.1177/11772719211066791

Abstract

Background: The anti-DFS70 autoantibodies are one of the most commonly and widely described agent of unknown clinical significance, frequently detected in healthy individuals. It is not known whether the DFS70 autoantibodies are protective or pathogenic. One of the factors suspected of inducing the formation of anti-DFS70 antibodies is increased oxidative stress. We evaluated the coexistence of anti-DFS70 antibodies with selected markers of oxidative stress and investigated whether these antibodies could be considered as indirect markers of oxidative stress.

Methods: The intensity of oxidative stress was measured in all samples via indices of free-radical damage to lipids and proteins such as total oxidant status (TOS), concentrations of lipid hydroperoxides (LPH), lipofuscin (LPS), and malondialdehyde (MDA). The parameters of the non-enzymatic antioxidant system, such as total antioxidant status (TAS) and uric acid concentration (UA), were also measured, as well as the activity of superoxide dismutase (SOD). Based on TOS and TAS values, the oxidative stress index (OSI) was calculated. All samples were also tested with indirect immunofluorescence assay (IFA) and 357 samples were selected for direct monospecific anti DFS70 enzyme-linked immunosorbent assay (ELISA) testing.

Results: The anti-DFS70 antibodies were confirmed by ELISA test in 21.29% of samples. Compared with anti-DFS70 negative samples we observed 23% lower concentration of LPH (P = .038) and 11% lower concentration of UA (P = .005). TOS was 20% lower (P = .014). The activity of SOD was up to 5% higher (P = .037). The Pearson correlation showed weak negative correlation for LPH, UA, and TOS and a weak positive correlation for SOD activity.

Conclusion: In samples positive for the anti-DFS70 antibody a decreased level of oxidative stress was observed, especially in the case of samples with a high antibody titer. Anti-DFS70 antibodies can be considered as an indirect marker of reduced oxidative stress or a marker indicating the recent intensification of antioxidant processes.

Keywords: Antibodies; biomarkers; free radicals; oxidative stress.

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Conflict of interest statement

Declaration of conflicting interests: The author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: Jacek J. Jóźwiak and Maciej Banach have received an unrestricted educational grant from Valeant, and has served as a consultant or speaker for Valeant. Paweł Krzemień is employed by Euroimmun Polska Sp. z o.o. (Wroclaw, Poland), whose autoantibody reagents were used in the study. Peter E. Penson owns 4 shares in AstraZeneca PLC and has received honoraria and/or travel reimbursement for events sponsored by AKCEA, Amgen, AMRYT, Link Medical, Mylan, Napp, Sanofi. All others authors declare no conflicts of interest concerning the results of this analysis.

Figures

**Figure 1.**
Research procedural stages.

**Figure 2.**
ANA AC-2 pattern distribution depending on the titer.

See this image and copyright information in PMC

References

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Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress

Affiliations

Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

References

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Full Text Sources