Corrigendum: Inhibition of Lung Tumor Development in ApoE Knockout Mice via Enhancement of TREM-1 Dependent NK Cell Cytotoxicity

Abstract

[This corrects the article DOI: 10.3389/fimmu.2019.01379.].

Keywords: Lung tumor development; NK cells; T-bet; TREM-1; apolipoprotein E.

Figure 3

Effect of ApoE knockdown on cell growth and migration in cancer cells. (A) Lung cancer cells (A549 and NCI-H460) and B16F10 cells were plated on 96-well plates (1x10³ cells per well) and transfected with the negative control (NC) siRNA or ApoE siRNA (25, 50 or 100 nM) for indicated time points. Cell growth was measured by MTT assay (n=5). (B) B16F10 cells were seeded on μ-Slide and transfected with NC siRNA or ApoE siRNA (50 or 100 nM). Cells were cultured to confluent on μ-Slide (n=3). After silicon-wall removal, cells were allowed to migrate into cell-free zone. Cell migration was detected at various times post-silicon-wall removal by a microscopy at 100X. (C) B16F10 cells transfected with NC siRNA or ApoE siRNA (50 or 100 nM) were seeded onto transwell inserts pre-coated with collagen on the bottom side and loaded into culture well filled with growth medium containing 10% FBS as a chemoattractant (n=3). After 18 h incubation, transwell inserts were fixed and stained by crystal violet solution. Bar graphs represent cell-migration distance or number of migrated cells. *p < 0.05 and ***p < 0.0001. (D) Cells were transfected with NC siRNA or ApoE siRNA (100 nM) for 24 h. Cell extracts were analyzed by Western blotting. Samples (30 μg) were resolved on SDS–PAGE and detected with specific antibodies against CDK4, CDK6, Cyclin D1, MMP-2, MMP-9 and ApoE. β-actin was used as a loading control.