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Comparative Study
. 1986 Feb 11;14(3):1355-64.
doi: 10.1093/nar/14.3.1355.

Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes

A Das, S Stachel, P Ebert, P Allenza, A Montoya, E Nester
Free PMC article
Comparative Study

Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes

A Das et al. Nucleic Acids Res. .
Free PMC article

Abstract

The DNA sequences of the promoter and 5' upstream regions of six Agrobacterium tumefaciens Ti-plasmid encoded virulence (vir) genes were determined. The transcription initiation sites were mapped by the S1 nuclease protection assay. In the -10 region, the vir promoters share a consensus sequence that is homologous to a DNA sequence found in the same region of E. coli promoters. In contrast, the -35 region sequences are variable. Several vir genes contain two common hexanucleotide sequences, 5'CGAGTA3' and 5'GCAATT3'. Translation initiation codons for all vir genes, except virG, are preceded by sequences homologous to the ribosome binding site sequences found in E. coli.

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