doi: 10.1073/pnas.83.5.1227.
Specific inhibition of mRNA translation by complementary oligonucleotides covalently linked to intercalating agents
- PMID: 3513172
- PMCID: PMC323048
- DOI: 10.1073/pnas.83.5.1227
Item in Clipboard
Specific inhibition of mRNA translation by complementary oligonucleotides covalently linked to intercalating agents
Proc Natl Acad Sci U S A.
1986 Mar.
Abstract
Synthetic oligodeoxynucleotides that are covalently linked at their 3' end to an acridine derivative and are complementary to the repeated sequence UUAAAUUAAAUUAAA adjacent to the ribosome binding site of the gene 32-encoded mRNA from phage T4 have been used to regulate the synthesis of gene 32-encoded protein in vitro. These modified, synthetic oligonucleotides specifically block the translation of gene 32-encoded mRNA with a higher efficiency than the homologous unsubstituted oligonucleotides. The inhibition produced by these short "anti-messengers" is due to the formation of specific mRNA . oligodeoxynucleotide hybrids that are stabilized by the intercalation of the acridine ring in the RNA . DNA duplex.
Similar articles
-
Oligodeoxynucleotides covalently linked to intercalating agents: a new class of gene regulatory substances.Biochimie. 1985 Jul-Aug;67(7-8):777-83. doi: 10.1016/s0300-9084(85)80167-x. Biochimie. 1985. PMID: 3910111
-
Enzymatic amplification of translation inhibition of rabbit beta-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents.Nucleic Acids Res. 1987 Jun 25;15(12):4717-36. doi: 10.1093/nar/15.12.4717. Nucleic Acids Res. 1987. PMID: 3037483 Free PMC article.
-
Nucleic acid-binding molecules with high affinity and base sequence specificity: intercalating agents covalently linked to oligodeoxynucleotides.Proc Natl Acad Sci U S A. 1984 Jun;81(11):3297-301. doi: 10.1073/pnas.81.11.3297. Proc Natl Acad Sci U S A. 1984. PMID: 6587350 Free PMC article.
-
Control of gene expression by oligonucleotides covalently linked to intercalating agents.Genome. 1989;31(1):413-21. doi: 10.1139/g89-062. Genome. 1989. PMID: 2687107 Review.
-
[Mechanism for suppression mRNA translation with antisense oligonucleotides].Mol Biol (Mosk). 1990 Sep-Oct;24(5):1157-61. Mol Biol (Mosk). 1990. PMID: 2290415 Review. Russian.
Cited by
-
Induction of chronic human immunodeficiency virus infection is blocked in vitro by a methylphosphonate oligodeoxynucleoside targeted to a U3 enhancer element.J Virol. 1991 Jan;65(1):213-9. doi: 10.1128/JVI.65.1.213-219.1991. J Virol. 1991. PMID: 1702158 Free PMC article.
-
Sequence dependent effects in methylphosphonate deoxyribonucleotide double and triple helical complexes.Nucleic Acids Res. 1990 Jun 25;18(12):3545-55. doi: 10.1093/nar/18.12.3545. Nucleic Acids Res. 1990. PMID: 2362807 Free PMC article.
-
Comparative inhibition of chloramphenicol acetyltransferase gene expression by antisense oligonucleotide analogues having alkyl phosphotriester, methylphosphonate and phosphorothioate linkages.Nucleic Acids Res. 1987 Jul 24;15(14):5749-63. doi: 10.1093/nar/15.14.5749. Nucleic Acids Res. 1987. PMID: 3475677 Free PMC article.
-
Selective inhibition of the cytopathic effect of type A influenza viruses by oligodeoxynucleotides covalently linked to an intercalating agent.Nucleic Acids Res. 1987 Dec 10;15(23):9909-19. doi: 10.1093/nar/15.23.9909. Nucleic Acids Res. 1987. PMID: 3697085 Free PMC article.
-
The common 5' terminal sequence on trypanosome mRNAs: a target for anti-messenger oligodeoxynucleotides.Nucleic Acids Res. 1986 Jul 25;14(14):5605-14. doi: 10.1093/nar/14.14.5605. Nucleic Acids Res. 1986. PMID: 3737413 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
