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. 2022 Jan 29:2022:1062261.
doi: 10.1155/2022/1062261. eCollection 2022.

Effects of the Chinese Herb Medicine Formula "She-Xiang-Yu-Hong" Ointment on Wound Healing Promotion in Diabetic Mice

Qingjie Li  1 , Xinjun Liu  1 , Shihui Yang  2 , Chunrun Li  2 , Wei Jin  1 , Weiwei Hou  1
Affiliations

Effects of the Chinese Herb Medicine Formula "She-Xiang-Yu-Hong" Ointment on Wound Healing Promotion in Diabetic Mice

Qingjie Li et al. Evid Based Complement Alternat Med. .

Abstract

Wound healing in diabetic patients is a difficult problem to be solved at present. In addition, patients with diabetes have an increased risk of postoperative wound complications. "She-Xiang-Yu-Hong" (SXYH) ointment is a type of traditional Chinese medicine (TCM) compound used to treat wounds. Over the past few years, SXYH has been applied in the Affiliated Hospital of Chengdu University of TCM (Chengdu, China) for the treatment of diabetic foot infections and bedsores, whereas there has been rare research on the effect of SXYH ointment on wound healing. In this study, SXYH ointment was first applied to streptozotocin (STZ)-triggered diabetic ICR mice (4-6 weeks, 20 ± 2 g) to observe the accelerated wound healing and the shortened wound healing period. As indicated by the histology and biochemistry analyses of skin biopsies, the wounds treated using SXYH ointment showed an increase in the granulation tissue. Moreover, SXYH also modulated the inflammation response by regulating affinity proinflammatory cytokines release (e.g., IL-6 and TNF-α). Furthermore, SXYH ointment obviously improved collagen fiber deposition and tissue on the wound surface. On the whole, this study indicated that SXYH ointment could accelerate wound healing, promote blood vessel formation, and suppress inflammations. Thus, the clinical potential of SXYH ointment was demonstrated in the treatment of diabetes and refractory wounds.

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Conflict of interest statement

The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1
Figure 1
Schematic illustration of the topical therapy with “She-Xiang-Yu-Hong” ointment on wound healing promotion in diabetic mice.
Figure 2
Figure 2
Topical application of SXYH ointment accelerates wound closure in full-thickness excisional wounds. (a) Representative photographs taken from full-thickness wounds of mice. (b) Rate of wound closure induced by topical application of ointment base (the vehicle control group) and SXYH ointment at days 3, 7, 14, and 21. Data are expressed as percentage of reduction area from the original wound size (day 0). Values are mean ± SD (n = 8 wounds/group) (∗P < 0.05).
Figure 3
Figure 3
(a) The effect of the ointment on epithelium proliferation and collagen deposition in excision wounds with full thicknesses by H&E and Masson's trichrome staining (40×). (b) Quantitative analysis of granulation tissue thickness after 7 days' wounding. (c) Quantitative analysis of epithelial crawling distance after 7 days' wounding (∗P < 0.05).
Figure 4
Figure 4
Effects of SXYH ointment in cytokines production in the skin wound biopsies Tissue homogenates were prepared from the wound biopsies obtained from animals at days 7 and 14 that were treated with SXYH ointment and with the vehicle control. (a) TNF-α and (b) IL-6 were assayed by ELISA. Data are mean ± SD (n = 8 wounds/group). ∗P < 0.05 compared to the vehicle control group.
Figure 5
Figure 5
The SXYH ointment rapidly advances angiogenesis in the wounds as compared to the vehicle control. Sections of wounds harvested on days 7, 14, and 21 postwounding were immunostained with anti-CD31 (a) or with anti-VEGF (b).
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