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. 2022 Feb 8;12(1):2141.
doi: 10.1038/s41598-022-06193-9.

Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Muhammad Shakeel  1 , Juan Du  1 , Shang-Wei Li  2 , Yuan-Jin Zhou  1 , Naeem Sarwar  3 , Xiaolan Guo  4
Affiliations

Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Muhammad Shakeel et al. Sci Rep. .

Abstract

Parental RNAi (pRNAi) is a response of RNA interference in which treated insect pests progenies showed a gene silencing phenotypes. pRNAi of CmGNA gene has been studied in Cnaphalocrocis medinalis via injection. Our results showed significant reduction in ovulation per female that was 26% and 35.26% in G1 and G2 generations, respectively. Significant reduction of hatched eggs per female were observed 23.53% and 45.26% as compared to control in G1-G2 generations, respectively. We also observed the significant variation in the sex ratio between female (40% and 53%) in G1-G2 generations, and in male (65%) in G1 generation as compared to control. Our results also demonstrated the significant larval mortality (63% and 55%) and pupal mortality (55% and 41%), and significant reduction of mRNA expression level in G1 and G2 generations. Our findings have confirmed that effectiveness of pRNAi induced silencing on the CmGNA target gene in G1-G2 generations of C. medinalis. These results suggested the potential role of pRNAi in insect pest resistance management strategies.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
The percentage laid eggs per female in G1–G3 generations. Each point indicates the mean ± standard error in G1–G3 generations along with their control groups. Significant differences indicated by *(P < 0.05), **(P < 0.01).
Figure 2
Figure 2
The percentage of hatched eggs per female were observed in G1–G3 generations. Each point indicates the mean ± standard error in G1–G3 generations and their control groups. Significant differences indicated by *(P < 0.05), **(P < 0.01).
Figure 3
Figure 3
The percentage of larval mortalities were observed in G1–G3 generations. Each point indicates the mean ± standard error in G1–G3, and their control groups. Significant differences indicated by *(P < 0.05), **(P < 0.01).
Figure 4
Figure 4
The percentage of dead pupae were calculated in G1–G3 generations. Each point indicates the mean ± standard error in G1–G3, and their control groups. Significant differences indicated by *(P < 0.05), **(P < 0.01).
Figure 5
Figure 5
The percentage of emerged males from pupation chamber were calculated in G1–G3 genrations. Each point indicates the mean ± standard error from G1–G3 generations, and their control groups. Significant differences indicated by *(P < 0.05), **(P < 0.01).
Figure 6
Figure 6
The percentage of emerged females after pupation. Each point indicates the mean ± standard error in G1–G3, and their control groups. Significant differences indicated by *(P < 0.05), **(P < 0.01).
Figure 7
Figure 7
The phenotypic deformities were evaluated from larvae and pupae in G1–G3 generations. Infected larvae were observed in G1–G3 generations of treated insects using pRNAi.
Figure 8
Figure 8
Pupae of treated insects exhibiting deformities in G1–G3 generations.
Figure 9
Figure 9
Changes in mRNA transcript level of CmGNA gene in G1–G3 generations after pRNAi. Each bar indicated the mean ± SD, and significant differences indicated by *(P < 0.05), **(P < 0.01).
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