Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Aug;15(4):912-924.
doi: 10.1007/s12602-022-09923-7. Epub 2022 Feb 9.

Effects of Dietary Supplementation with Lactobacillus acidophilus and Bacillus subtilis on Mucosal Immunity and Intestinal Barrier Are Associated with Its Modulation of Gut Metabolites and Microbiota in Late-Phase Laying Hens

Xin Chen  1 , Weiwen Chen  1 , Wenjia Ci  1 , Yingying Zheng  1 , Xinyan Han  2 , Jianping Huang  3 , Jianjin Zhu  4
Affiliations

Effects of Dietary Supplementation with Lactobacillus acidophilus and Bacillus subtilis on Mucosal Immunity and Intestinal Barrier Are Associated with Its Modulation of Gut Metabolites and Microbiota in Late-Phase Laying Hens

Xin Chen et al. Probiotics Antimicrob Proteins. 2023 Aug.

Abstract

We investigated the effects of dietary supplementation with Lactobacillus acidophilus and Bacillus subtilis on the intestinal immune response, intestinal barrier function, cecal microbiota profile, and metabolite profile in late-phase laying hens. Hens were divided into three groups and fed with the basal diet (NC group), basal diet supplementation with 250 mg/kg B. subtilis and L. acidophilus mixture powder (LD group), and basal diet supplementation with 500 mg/kg B. subtilis and L. acidophilus mixture powder (HD group), respectively. The results indicated that the dietary supplementation with L. acidophilus and B. subtilis increased the integrity of the intestinal barrier as evidenced by the significant increase in the number of ileal goblet cells and improve the expression of occludin, claudin-1, and ZO-1 genes in the HD group. Moreover, the levels of IL-6, TNF-α, and IFN-γ were significantly decreased in the LD and HD groups. The levels of immunoglobulin G (IgG) increased in the LD and HD group, and the levels of secretory immunoglobulin A (sIgA) increased with the HD treatment. Furthermore, 16 s rRNA sequencing revealed L. acidophilus in combination with B. subtilis increased the diversity of gut microbiota. The metabolomic analysis revealed beneficial changes in the amino acid metabolism and lipid metabolism (decrease in LysoPC and LysoPE levels). In conclusion, dietary supplementation with L. acidophilus and B. subtilis could improve intestinal barrier function and maintain immune homeostasis. These beneficial effects may be associated with the modulation of the intestinal microbiome and metabolites.

Keywords: Immune responses; Intestinal barrier; Late-phase laying hens; Metabolomics; Microbiota; Probiotics.

PubMed Disclaimer

References

    1. Ge YT, Lin SM, Li BW, Yang YH, Tang X, Shi YH, Sun J, Le GW (2020) Oxidized pork induces oxidative stress and inflammation by altering gut microbiota in mice. Mol Nutr Food Res 64(2):e1901012. https://doi.org/10.1002/mnfr.201901012 - DOI - PubMed
    1. Li X, Mao MY, Zhang YN, Yu KF, Zhu WY (2019) Succinate modulates intestinal barrier function and inflammation response in pigs. Biomolecules 9(9):486. https://doi.org/10.3390/biom9090486 - DOI - PubMed - PMC
    1. Bron PA, Kleerebezem M, Brummer RJ, Cani PD, Mercenier A, MacDonald TT, Garcia-Rodenas CL, Wells JM (2017) Can probiotics modulate human disease by impacting intestinal barrier function? Br J Nutr 117(1):93–107. https://doi.org/10.1017/S0007114516004037 - DOI - PubMed - PMC
    1. Paone P, Cani PD (2020) Mucus barrier, mucins and gut microbiota: the expected slimy partners? Gut 69(12):2232–2243. https://doi.org/10.1136/gutjnl-2020-322260 - DOI - PubMed
    1. Honda K, Littman DR (2016) The microbiota in adaptive immune homeostasis and disease. Nature 535(7610):75–84. https://doi.org/10.1038/nature18848 - DOI - PubMed

Publication types

LinkOut - more resources