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. 2022 May 16;61(21):e202117655.
doi: 10.1002/anie.202117655. Epub 2022 Feb 21.

Trapping-Enrichment Multi-dimensional Liquid Chromatography with On-Line Deuterated Solvent Exchange for Streamlined Structure Elucidation at the Microgram Scale

Imad A Haidar Ahmad  1 , Gioacchino Luca Losacco  1 , Vladimir Shchurik  1 , Xiao Wang  1 , Ryan D Cohen  1 , Alastair N Herron  2 , Sheenagh Aiken  3 , Daniele Fiorito  3 , Heather Wang  1 , Mikhail Reibarkh  1 , Timothy Nowak  1 , Alexey A Makarov  1 , Dwight R Stoll  4 , Davy Guillarme  5   6 , Ian Mangion  1 , Varinder K Aggarwal  3 , Jin-Quan Yu  2 , Erik L Regalado  1
Affiliations

Trapping-Enrichment Multi-dimensional Liquid Chromatography with On-Line Deuterated Solvent Exchange for Streamlined Structure Elucidation at the Microgram Scale

Imad A Haidar Ahmad et al. Angew Chem Int Ed Engl. .

Abstract

At the forefront of chemistry and biology research, development timelines are fast-paced and large quantities of pure targets are rarely available. Herein, we introduce a new framework, which is built upon an automated, online trapping-enrichment multi-dimensional liquid chromatography platform (TE-Dt-mDLC) that enables: 1) highly efficient separation of complex mixtures in a first dimension (1 D-UV); 2) automated peak trapping-enrichment and buffer removal achieved through a sequence of H2 O and D2 O washes using an independent pump setup; and 3) a second dimension separation (2 D-UV-MS) with fully deuterated mobile phases and fraction collection to minimize protic residues for immediate NMR analysis while bypassing tedious drying processes and minimizing analyte degradation. Diverse examples of target isolation and characterization from organic synthesis and natural product chemistry laboratories are illustrated, demonstrating recoveries above 90 % using as little as a few micrograms of material.

Keywords: Analytical Methods; Liquid Chromatography; Mass Spectrometry; Multicomponent Isolation; Structure Elucidation.

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