Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2022 Jan 31;14(1):e21766.
doi: 10.7759/cureus.21766. eCollection 2022 Jan.

Flow-Cytometry in the Diagnosis of Diffuse Large B-Cell Lymphoma Based on Stomach Tissue Samples: A Case Report

Tung Tuan Nguyen  1 , An Thi Vinh Do  1 , Nga Thi Nguyen  2 , Thanh Quoc Truong  3 , Ai Thuc Ton  1
Affiliations
Case Reports

Flow-Cytometry in the Diagnosis of Diffuse Large B-Cell Lymphoma Based on Stomach Tissue Samples: A Case Report

Tung Tuan Nguyen et al. Cureus. .

Abstract

Morphology and immunohistochemistry on node, tissue, and bone marrow biopsies are frequently used in lymphoma diagnosis to characterize the stage and subtype of diseases. Multicolor flow cytometry technology is a novel technique for the analysis of immunological markers to identify lymphoma on fresh tissue when immunohistochemical staining is ambiguous. We report a case of a patient diagnosed with diffuse large B-cell lymphoma by flow cytometry on a stomach tissue biopsy.

Keywords: diffuse large b-cell lymphoma; flow cytometry; fresh tissue; immunohistochemistry; lymphoma.

PubMed Disclaimer

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. IHC of CD20+ in bone marrow (a); stomach(b); node(c)
Figure 2
Figure 2. IHC of CD3+ in bone marrow(a); node(b); stomach(c)
Figure 3
Figure 3. IHC of Ki67+ in bone marrow(a); node(b); stomach(c)
Figure 4
Figure 4. Flow cytometry on fresh tissue from stomach biopsy
(a): Lymphocytes (green dots) accounted for 85,1%; (b): Lymphocytes (green dots) positive for cyCD3 (73,7%), positive for CD79a (23,1%); (c): positive for CD4, CD8 (Navios EX - Beckman Coulter)
Figure 5
Figure 5. Flow cytometry on fresh tissue from stomach biopsy samples
(d): Lymphocytes (green dots) positive for CD20, CD38; (e): positive for CD81, negative for CD23; (f): negative for kappa, lambda (Navios EX - Beckman Coulter)
See this image and copyright information in PMC

References

    1. A clinical evaluation of the International Lymphoma Study Group classification of non-Hodgkin's lymphoma. The Non-Hodgkin's Lymphoma Classification Project. http://10.1182/blood.V89.11.3909. Blood. 1997;89:3909–3918. - PubMed
    1. EBV-positive diffuse large B-cell lymphoma of the elderly is an aggressive post-germinal center B-cell neoplasm characterized by prominent nuclear factor-kB activation. Montes-Moreno S, Odqvist L, Diaz-Perez JA, et al. Mod Pathol. 2012;25:968–982. - PubMed
    1. EBV-positive diffuse large B-cell lymphoma of the elderly: a case series from Peru. Beltran BE, Castillo JJ, Morales D, et al. Am J Hematol. 2011;86:663–667. - PubMed
    1. Epidemiology of the non-Hodgkin's lymphomas: distributions of the major subtypes differ by geographic locations. Non-Hodgkin's Lymphoma Classification Project. Anderson JR, Armitage JO, Weisenburger DD. Ann Oncol. 1998;9:717–720. - PubMed
    1. Impact of concordant and discordant bone marrow involvement on outcome in diffuse large B-cell lymphoma treated with R-CHOP. Sehn LH, Scott DW, Chhanabhai M, et al. J Clin Oncol. 2011;29:1452–1457. - PubMed

Publication types

LinkOut - more resources