The complete mitochondrial genome data of the Common Rose butterfly, Pachliopta aristolochiae (Lepidoptera, Papilionoidea, Papilionidae) from Malaysia

Abstract

Here, we present the complete mitochondrial genome of Pachliopta aristolochiae, a Common Rose butterfly from Malaysia. The sequence was generated using Illumina NovaSeq 6000 sequencing platform. The mitogenome is 15,235bp long, consisting of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two D-loop regions. The total base composition was (81.6%), with A (39.3%), T (42.3%), C (11.0%) and G (7.3%). The gene order of the three tRNAs was trnM-trnI-trnQ, which differs from the ancestral insect gene order trnI-trnQ-trnM. Phylogenetic tree analysis revealed that the sequenced Pachliopta aristolochiae in this data is closely related to Losaria neptunus (NC 037868), with highly supported ML and BI analysis. The data presented in this work can provide useful resources for other researchers to study deeper into the phylogenetic relationships of Lepidoptera and the diversification of the Pachliopta species. Also, as one of the bioindicator species, this data can be used to assess environmental changes in the terrestrial and aquatic ecosystem via enviromental DNA approahes. The mitogenome of Pachliopta aristolochiae is available in GenBank under the accession number MZ781228.

Keywords: Lepidoptera; Malaysia; Mitogenome; Pachliopta aristolochiae; Papilionidae.

Fig. 1

Mitogenome map of Pachliopta aristolochiae generated using OGDRAW . The genes scattered on the heavy strand are shown on the outer side of the circle, while the inner side shows those that are scattered on the light strand. The arrows indicate the direction of gene transcription. CR represents the control region (D-loop).

Fig. 2

Features of the two D-loop regions of Pachliopta aristolochiae mitogenome located between trnS1 and trnE, as well as 12S rRNA and trnM. Conserved motifs ‘ATAGA’ and ‘ATTTA’ are indicated in red and blue respectively. Poly-T stretch is indicated in green while microsatellite-like elements (TA)n and (AT)n are shown in yellow.

Fig. 3

Phylogenetic tree of Pachliopta aristolochiae (MZ781228), indicated by asterisk (*) and 21 other Lepidoptera mitogenomes built using Maximum-Likelihood (ML) and Bayesian Inference (BI) approach. Bootstrap support values were indicated on each tree node, showing the results of ML and BI analysis. Caerulea coeligena (NC 058607) and Shijimiaeoides divina (NC 029763) from the family Lycaenidae were used as outgroups.