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. 2022 May;77(5):1610-1613.
doi: 10.1111/all.15251. Epub 2022 Feb 16.

IL-33 prevents the enhancement of AP-N, DPP4, and ACE2 expression induced by rhinovirus HRV16 in the human lung endothelium-potential implications for coronaviral airway infections

Izabela Gulbas  1 , Adrian Gajewski  1 , Mateusz Gawrysiak  1 , Robert Szewczyk  1 , Aleksandra Likońska  1 , Sylwia Michlewska  2 , Marek L Kowalski  1 , Maciej Chałubiński  1
Affiliations

IL-33 prevents the enhancement of AP-N, DPP4, and ACE2 expression induced by rhinovirus HRV16 in the human lung endothelium-potential implications for coronaviral airway infections

Izabela Gulbas et al. Allergy. 2022 May.
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIGURE 1
FIGURE 1
mRNA and protein expression in HMVEC‐L cells incubated with HRV16 (MOI3) alone or upon 24‐h pre‐stimulation with IL‐33 (10 ng/ml) or stimulated only with IL‐33 (10 ng/ml)up to 72 h after infection. (A) The effect of HMVEC‐L pre‐stimulation with IL‐33 (10 ng/ml) on HRV16 copies. (B, C) Release of IFN‐β and RANTES by HRV16‐infected HMVEC‐L. (D)AP‐N mRNA expression and (E, F) AP‐N surface expression (green—AP‐N‐FITC, blue nuclei—DAPI) (confocal microscope) (G) DPP4mRNA expression and (H, I)DPP4surfaceexpression(red—DPP4—Alexa Fluor 594, blue nuclei—DAPI) (confocal microscope). The Mann–Whitney U‐test was used to analyze differences between the two groups. Data are representatives of at least four independent experiments as means ± SEM; n = 6–10; *p < 0.05; **p < 0.01; ***p < 0.001 as compared to mock cells, #p < 0.05; ##p < 0.01; ###p < 0.001 as compared to HRV16‐induced cells
FIGURE 2
FIGURE 2
mRNA and protein expression in HMVEC‐L cells incubated with HRV16 (MOI3) alone or upon cell 24‐h pre‐stimulation with IL‐33 (10 ng/ml) or stimulated only with IL‐33 (10 ng/ml) up to 72 h after infection. (A) ACE2mRNA expression (B, C) ACE2 surface expression(red—ACE2—Alexa Fluor 594, blue nuclei—DAPI) (confocal microscope) (D, E) TMPRSS2surfaceexpression(green—TMPRSS2‐FITC, blue nuclei—DAPI) (confocal microscope) (F)ICAM‐1mRNA expression(G,H)ICAM‐1 surface expression (red—ICAM‐1‐PE, blue nuclei—DAPI) (confocal microscope). The Mann–Whitney U‐test was used to analyze differences between the two groups. Data are representatives of at least four independent experiments as means ± SEM; n = 6–10; *p < 0.05; **p < 0.01; ***p < 0.001 as compared to mock cells, #p < 0.05; ##p < 0.01; ###p < 0.001 as compared to HRV16‐induced cells
See this image and copyright information in PMC

References

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