Complete genome sequence of Shewanella algae strain 2NE11, a decolorizing bacterium isolated from industrial effluent in Peru

Abstract

Shewanella is a microbial group with high potential to be applied in textile effluents bioremediation due to its ability to use a wide variety of substrates as a final electron acceptor in respiration. The present research aimed to describe a new strain, Shewanella algae 2NE11, a decolorizing bacterium isolated from industrial effluent in Peru. S. algae 2NE11 showed an optimal growth under pH 6-9, temperature between 30-40 °C, and 0-4 % NaCl. It can tolerate high concentrations of NaCl until 10% and low temperatures as 4 °C. It decolorizes azo and anthraquinone dyes with a decolorization rate of 89-97%. We performed next-generation sequencing (Pacific Bioscience®) and achieved its complete genome sequence with a length of 5,030,813bp and a GC content of 52.98%. Genomic characterization revealed the presence of protein-coding genes related to decolorization like azoreductase, dyp-peroxidase, oxidoreductases, and the complete Mtr respiratory pathway. Likewise, we identified other properties such as the presence of metal resistant genes, and genes related to lactate and N-acetylglucosamine metabolism. These results highlight its potential to be applied in the bioremediation of textile effluents and guide future research on decolorization metabolic pathways.

Keywords: Shewanella algae; decolorization; dyes; whole-genome sequencing.

Figure 1

Decolorization kinetic by S. algae 2NE11. The graph shows the decolorization of Methyl Orange (●), Bright Blue Remazol (■), Yellow Procion HEXL (▲), Direct Blue 71 (♦), and control (*). Each measure in the kinetic contains a standard deviation bar.

Figure 2

A. Visualization of S. algae 2NE11 genome compared with S. algae KC-Na-R1 (NZ_CP033575.1) and S. algae CCU101 (NZ_CP018456.1). It shows genomic island prediction in two positions (GI-I and GI-II). Starting from the inner circle moving outwards, the following tracks are shown: GC content (Black), GC skew– (Purple), GC skew+ (Green), S. algae KC-Na-R1 genome (light blue), S. algae CCU101 (ed). B. Bar plot showing the principal functional categories of strain 2NE11 according to the KEGG Orthology is depicted.