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. 2022:29:8.
doi: 10.1051/parasite/2022009. Epub 2022 Feb 11.

Genetic interrelationships of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidea), the causative agent of sparganosis in Europe

Eva Čisovská Bazsalovicsová  1 , Alžbeta Radačovská  1 , Antti Lavikainen  2 , Roman Kuchta  3 , Ivica Králová-Hromadová  1
Affiliations

Genetic interrelationships of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidea), the causative agent of sparganosis in Europe

Eva Čisovská Bazsalovicsová et al. Parasite. 2022.

Abstract
in English, French

The geographic distribution of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidea), the causative agent of food/water-borne sparganosis, is restricted to Europe, where infected canids, felids, mustelids, suids, and reptiles have been documented from Poland, Ukraine, Belarus, Russia, Serbia, Estonia, Latvia, and Finland. The main objective of the current study was to map the molecular divergence of S. erinaceieuropaei from Finland using the complete sequences of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1 mtDNA). Seven cox1 haplotypes were determined in 15 tapeworms from Eurasian lynx (Lynx lynx) from three localities in southern Finland. In addition, the first inter-population study of S. erinaceieuropaei based on currently obtained data on cox1 from Finland and previously published data from Finland, Latvia, Ukraine, and Poland, was performed. The haplotype network showed a star-like pattern without specific subdivision of lineages according to the locality. Samples from Finland, Latvia, and Poland shared several haplotypes and formed the common Baltic lineage. The haplotype of S. erinaceieuropaei from Ukraine was unique and placed on a separate mutational pathway, suggesting a different lineage of the parasite.

Title: Interrelations génétiques de Spirometra erinaceieuropaei (Cestoda, Diphyllobothriidea), l’agent causal de la sparganose en Europe.

Abstract: La distribution géographique de Spirometra erinaceieuropaei (Cestoda : Diphyllobothriidea), l’agent causal de la sparganose d’origine alimentaire/hydrique, est limitée à l’Europe, où des canidés, félidés, mustélidés, suidés et reptiles infectés ont été documentés en Pologne, Ukraine, Biélorussie, Russie, Serbie, Estonie, Lettonie et Finlande. L’objectif principal de la présente étude était de cartographier la divergence moléculaire de S. erinaceieuropaei de Finlande à l’aide des séquences complètes du gène mitochondrial de la sous-unité 1 de la cytochrome c oxydase (ADNmt cox1). Sept haplotypes cox1 ont été déterminés chez quinze cestodes du Lynx d’Eurasie (Lynx lynx) de trois localités du sud de la Finlande. En outre, la première étude inter-populationnelle de S. erinaceieuropaei basée sur les données actuellement obtenues sur cox1 de Finlande et sur des données précédemment publiées de Finlande, Lettonie, Ukraine et Pologne, a été réalisée. Le réseau d’haplotypes a montré un motif en étoile sans subdivision spécifique des lignées selon la localité. Des échantillons de Finlande, Lettonie et Pologne partagent plusieurs haplotypes et forment la lignée commune de la Baltique. L’haplotype de S. erinaceieuropaei d’Ukraine est unique et placé sur une voie de mutation distincte suggérant une lignée différente du parasite.

Keywords: Food/water-borne zoonosis; Genetic lineages; Molecular genotyping; Sparganosis in Finland; cox1 haplotypes.

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Figures

Figure 1
Figure 1
Scheme of the distribution of Spirometra erinaceieuropaei (S. e.) from Finland (FI), Poland (PL), Latvia (LV), and Ukraine (UA) analysed molecularly in the current work (blue points) and its findings from neighbouring Estonia (EE), Belarus (BY), and Ukraine (UA) based on its morphology (green points). The map was obtained from https://d-maps.com.
Figure 2
Figure 2
Haplotype network diagram based on mitochondrial cox1 haplotypes of Spirometra erinaceieuropaei from: (A) Finland (current data), Latvia [1], Poland [7, 14], and Ukraine [14]; and (B) specifically for Finland and Latvia. The sizes of haplotypes are proportional to the number of samples. Codes denote haplotype identifiers detected in individuals from Finland. Each hatch mark represents a single mutation, while black dots symbolise intermediate missing or unsampled haplotypes.
See this image and copyright information in PMC

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