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. 2022 Jun;26(6):4315-4325.
doi: 10.1007/s00784-022-04395-z. Epub 2022 Feb 11.

Chemical analysis of irradiated root dentin and its interaction with resin cements

Alessandro Marcelo Pelloso  1 , Rafael Resende de Miranda  1 , Matheus Elias Rossi  1 , Adriano Luiz Balthazar Bianchini  2 , Francisco Américo Silveira Marcelino  2 , Edna Lúcia Coutinho da Silva  3 , Veridiana Resende Novais  4
Affiliations

Chemical analysis of irradiated root dentin and its interaction with resin cements

Alessandro Marcelo Pelloso et al. Clin Oral Investig. 2022 Jun.

Abstract

Objectives: To investigate the chemical changes in root dentin submitted to ionizing radiation and how it affects the interaction with resin cements.

Materials and methods: Forty human premolars were randomly divided into two groups (n = 20): non-irradiated and irradiated. They were randomly subdivided according to the type of resin cement (n = 10): conventional (RelyX ARC, 3 M ESPE) or self-adhesive (RelyX U200, 3 M ESPE). After cementation of the fiberglass posts, the roots were sectioned to be analyzed by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, and confocal laser scanning microscopy (CLSM). The data obtained from FTIR and Raman were analyzed using two-way ANOVA followed Tukey's test (α = 0.05). For CLSM, a descriptive analysis was performed.

Results: In the FTIR, there was a significant difference between the non-irradiated and irradiated groups for phosphate (p = 0.011), carbonate (p < 0.001), amide III (p = 0.038), and carbonate/mineral ratio (p < 0.001). Regarding the root third, there was a difference for amide I (p = 0.002), mineral/matrix ratio (p = 0.001), and amide I/CH2 (p = 0.026) between the cervical and the others. Raman spectroscopy revealed no difference between groups for 961/1458 cm-1 in the diffusion zone. CLSM showed a different interaction pattern for the two cements with the irradiated dentin from the cervical third.

Conclusions: Ionizing radiation altered the chemical composition of root dentin, especially in the cervical third. The resin cements showed less interaction with the irradiated root dentin.

Clinical relevance: As radiotherapy alters the chemical composition of root dentin, the interaction of resin cement with dentin can compromise the success of rehabilitation with fiberglass posts.

Keywords: Dental bonding; Dentin; Fourier transform infrared; Radiotherapy; Resin cement; Spectroscopy.

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References

    1. Bhandari S, Soni BW, Bahl A, Ghoshal S (2020) Radiotherapy-induced oral morbidities in head and neck cancer patients. Spec Care Dentist 40(3):238–250. https://doi.org/10.1111/scd.12469 - DOI - PubMed
    1. Miranda RR, Lopes CCA, Franco NMAS, Cabral LC, Simamoto Júnior PC, Novais VR (2020) Head and neck cancer therapy-related oral manifestation management in the COVID-19 pandemic: a critical review. Braz Oral Res 34:e120. https://doi.org/10.1590/1807-3107bor-2020.vol34.0120 - DOI - PubMed
    1. Thiagarajan A, Iyer NG (2014) Radiation-induced sarcomas of the head and neck. World J Clin Oncol 5(5):973–981. https://doi.org/10.5306/wjco.v5.i5.973 - DOI - PubMed - PMC
    1. Jawad H, Hodson NA, Nixon PJ (2015) A review of dental treatment of head and neck cancer patients, before, during and after radiotherapy: part 1. Br Dent J 218(2):65–68. https://doi.org/10.1038/sj.bdj.2015.28 - DOI - PubMed
    1. Gupta N, Pal M, Rawat S, Grewal MS, Garg H, Chauhan D, Ahlawat P, Tandon S, Khurana R, Pahuja AK, Mayank M, Devnani B (2015) Radiation-induced dental caries, prevention and treatment - a systematic review. Natl J Maxillofac Surg 6(2):160–166. https://doi.org/10.4103/0975-5950.183870 - DOI - PubMed - PMC

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