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. 1986 Apr;83(7):2219-22.
doi: 10.1073/pnas.83.7.2219.

Detection of hepatitis B virus X product using an open reading frame Escherichia coli expression vector

E Elfassi, W A Haseltine, J L Dienstag

Detection of hepatitis B virus X product using an open reading frame Escherichia coli expression vector

E Elfassi et al. Proc Natl Acad Sci U S A. 1986 Apr.

Abstract

The genome of the hepatitis B virus (HBV) contains a sequence, designated X, capable of encoding a protein of 154 amino acids. To determine whether the putative protein synthesized from this region is antigenic, we examined the sera of HBV-infected patients for the ability to react with a hybrid protein that contained 133 amino acids encoded by the X region and portions of the bacterial ompF and beta-galactosidase genes. Some HBV-positive sera tested contained antibodies that specifically recognized the hybrid protein. All sera were from patients diagnosed as suffering from chronic active hepatitis. We conclude that the X region of HBV encodes a protein and that this protein is antigenic in some patients.

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