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. 2021 Dec 20;10(2):554-563.
doi: 10.1002/fsn3.2681. eCollection 2022 Feb.

Prevalence, characterization, and antibiotic susceptibility of Cronobacter spp. in a milk powder processing environment: The first reported case in Serbia

Csaba Csorba  1 , Marija Pajić  1 , Bojan Blagojević  1 , Stephen Forsythe  2 , Miodrag Radinović  1 , Branko Velebit  3
Affiliations

Prevalence, characterization, and antibiotic susceptibility of Cronobacter spp. in a milk powder processing environment: The first reported case in Serbia

Csaba Csorba et al. Food Sci Nutr. .

Abstract

Cronobacter spp. are opportunistic foodborne pathogens that most often infect neonates and infants through contaminated powdered infant formula. No reports have been published in Serbia on the prevalence of Cronobacter spp. in powdered milk production environments. Consequently, this study aimed to determine the prevalence, molecular characterization, antimicrobial susceptibility, and biofilm-forming ability of Cronobacter spp. isolated from a milk powder plant. Hundred samples were collected from the production facility. Fifteen Cronobacter sakazakii strains were isolated and identified, giving a contamination rate of 15%. Using multi-locus sequence typing, the isolates were divided into five sequence types (STs). Cronobacter sakazakii ST4 (50%), ST1 (16.67%), and ST83 (16.67%) were the dominant STs isolated. A novel sequence type (ST759) was identified and registered in the Cronobacter MLST database. The results of the antibiotic susceptibility testing indicated that C. sakazakii strains were susceptible to piperacillin/tazobactam, ampicillin/sulbactam, and amoxicillin/clavulanate, especially to meropenem and cefotaxime. Most of the ST4 showed moderate-to-strong biofilm-forming ability. The presence of clinically relevant isolates (ST4, ST1, ST83, and ST8) revealed that the production plant is likely a potential concern for public health. Finally, finding new sequence types like the one detected in this study (ST759) underlines evolving genetic changes in C. sakazakii.

Keywords: Cronobacter sakazakii; MLST; antimicrobial resistance; biofilm production; powdered milk; prevalence.

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Figures

FIGURE 1
FIGURE 1
Etest MICs of piperacillin/tazobactam (P/T), ampicillin/sulbactam (AB), amoxicillin/clavulanic acid (XL), meropenem (MP), and cefotaxime (CT) for an environmental isolate of Cronobacter sakazakii
See this image and copyright information in PMC

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