Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 May;5(5):1479-85.
doi: 10.1093/nar/5.5.1479.

Detection of 5-methylcytosine in DNA sequences

H Ohmori, J I Tomizawa, A M Maxam
Free PMC article

Detection of 5-methylcytosine in DNA sequences

H Ohmori et al. Nucleic Acids Res. 1978 May.
Free PMC article

Abstract

Col E1 DNA has methylated cytosine in the sequence 5'-CC*(A/T)GG-3' and methylated adenine in the sequence 5'-GA*TC-3' at the positions indicated by asterisks(*). When the Maxam-Gilbert DNA sequencing method is applied to this DNA, the methylated cytosine (5-methylcytosine) is found to be less reactive to hydrazine than are cytosine and thymine, so that a band corresponding to that base does not appear in the pyrimidine cleavage patterns. The existence of the methylated cytosine can be confirmed by analyzing the complementary strand or unmethylated DNA. In contrast, the methylated adenine (probably N6-methyladenine) cannot be distinguished from adenine with standard conditions for cleavage at adenine.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1964 Nov 15;91:462-76 - PubMed
    1. J Bacteriol. 1976 May;126(2):990-6 - PubMed
    1. J Mol Biol. 1977 Jul;114(1):153-68 - PubMed
    1. Nucleic Acids Res. 1976 Oct;3(10):2665-75 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 May;74(5):1865-9 - PubMed