Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 May;5(5):1701-13.
doi: 10.1093/nar/5.5.1701.

Replacement of wybutine by hydrazines and its effect on the active conformation of yeast tRNAPhe

H G Schleich, W Wintermeyer, H G Zachau
Free PMC article

Replacement of wybutine by hydrazines and its effect on the active conformation of yeast tRNAPhe

H G Schleich et al. Nucleic Acids Res. 1978 May.
Free PMC article

Abstract

The highly modified base wybutine (YWye) next to the anticodon of yeast tRNAPhe has been replaced by different hydrazine derivatives. The effect of the replacement on the activity of the tRNA has been studied in the heterologous aminoacylation with synthetase from E. coli and in the poly(U) directed binding to ribosomes from both yeast and E. coli. It was found that starting from tRNA-PheYWye the activity increased with increasing size, aromaticity, and stacking tendency of the substituent replacing YWye. It is concluded that YWye by the size of its aromatic system and by its stacking properties is particularly well suited for stabilizing the native conformation of tRNAPhe.

PubMed Disclaimer

References

    1. J Biol Chem. 1968 Feb 10;243(3):584-91 - PubMed
    1. Science. 1976 Nov 19;194(4267):796-806 - PubMed
    1. Eur J Biochem. 1972 Dec 4;31(2):215-20 - PubMed
    1. Biochim Biophys Acta. 1971 Jun 30;240(2):163-9 - PubMed
    1. Eur J Biochem. 1974 Apr 16;43(3):601-7 - PubMed