Stability of fluorescent antibody conjugates stored under various conditions

Abstract

Two experiments were carried out to determine the stability of fluorescent antibody conjugates. In experiment 1, Francisella tularemia conjugates in the lyophilized state retained their original staining titer for 1,294 days when stored at 25, 4 to 5, and -20 C; at 37 C the conjugates were stable for at least 65 days. In the liquid state at pH 7.4 and 8.0 these conjugates were stable for 1,294 days at 4 to 5 and -20 C, whereas those stored at 25 C remained stable through days 473 and 160 of storage, respectively, after which the staining titer gradually dropped. In experiment 2 five previously lyophilized conjugates were rehydrated with three different diluents and stored at 4 to 5 C for up to 600 days at their working dilutions. All of these conjugates retained their original staining titer during the test period except an anti-human globulin conjugate rehydrated with phosphate-buffered saline. Recommendations are made for the long-term storage of fluorescent antibody conjugates.