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. 2022 Jan 19;23(3):1088.
doi: 10.3390/ijms23031088.

Protein-Mediated Transformations of Superparamagnetic Nanoparticles Evidenced by Single-Particle Inductively Coupled Plasma Tandem Mass Spectrometry: A Disaggregation Phenomenon

Jacek Sikorski  1 , Magdalena Matczuk  1 , Agnieszka Kamińska  1 , Joanna Kruszewska  1 , Maciej Trzaskowski  2 , Andrei R Timerbaev  3 , Maciej Jarosz  1
Affiliations

Protein-Mediated Transformations of Superparamagnetic Nanoparticles Evidenced by Single-Particle Inductively Coupled Plasma Tandem Mass Spectrometry: A Disaggregation Phenomenon

Jacek Sikorski et al. Int J Mol Sci. .

Abstract

Progress toward translating superparamagnetic iron oxide nanoparticles (SPIONs) with specific diagnostic and therapeutic properties for clinical applications depends on developing and implementing appropriate methodologies that would allow in-depth characterizations of their behavior in a real biological environment. Herein, we report a versatile approach for studying interactions between SPIONs and proteins using single-particle inductively coupled plasma tandem mass spectrometry. By monitoring the changes in the size distribution upon exposure to human serum, the formation of stable protein corona is revealed, accompanied by particle disaggregation.

Keywords: human serum; single-particle inductively coupled plasma tandem mass spectrometry; superparamagnetic iron oxide nanoparticles.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
BF-STEM image (A) and magnetic properties (B) of synthesized nanoparticles.
Figure 2
Figure 2
Size distribution of original SPIONs ((A), orange), incubated with serum without ((A,B); black) and with ((A), blue) ultrafiltration, and incubated with albumin ((B), green), without ultrafiltration. The concentration of SPIONs in all suspensions—500 ng/L Fe.
See this image and copyright information in PMC

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