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. 2022 Feb 8;27(3):1133.
doi: 10.3390/molecules27031133.

Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles

Ismael Otri  1   2 , Serena Medaglia  1   2 , Elena Aznar  1   2   3   4 , Félix Sancenón  1   2   3   4   5 , Ramón Martínez-Máñez  1   2   3   4   5
Affiliations

Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles

Ismael Otri et al. Molecules. .

Abstract

Mesoporous silica nanoparticles loaded with rhodamine B and capped with curcumin are used for the selective and sensitive fluorogenic detection of human serum albumin (HSA). The sensing mesoporous silica nanoparticles are loaded with rhodamine B, decorated with aminopropyl moieties and capped with curcumin. The nanoparticles selectively release the rhodamine B cargo in the presence of HSA. A limit of detection for HSA of 0.1 mg/mL in PBS (pH 7.4)-acetonitrile 95:5 v/v was found, and the sensing nanoparticles were used to detect HSA in spiked synthetic urine samples.

Keywords: HSA detection; gated hybrid materials; mesoporous silica; sensors.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Scheme 1
Scheme 1
Schematic representation of the sensing mechanism of S1 nanoparticles in the presence of HSA.
Figure 1
Figure 1
(A) Powder X-ray diffraction patterns of (a) as-synthesized MSNs, (b) calcined MSNs and (c) solid S1. (B) TEM images of (d) calcined MSNs and (e) solid S1. (C) Z-potential of (f) calcined MSNs, (g) amino-functionalized and loaded MSNs and (h) solid S1. (D) Hydrodynamic diameter of calcined MSNs and S1.
Figure 2
Figure 2
Release of rhodamine B versus time from PBS (pH 7.4)-acetonitrile 95:5 v/v suspensions of S1 nanoparticles (a) in the absence and (b) in the presence of HSA (1000 µg mL−1) at 25 °C (100% is the maximum of rhodamine B released from S1 solid).
Figure 3
Figure 3
Calibration curve of S1 nanoparticles suspended in PBS (pH 7.4)-acetonitrile 95:5 v/v upon addition of increasing concentrations of HSA (0.1 to 100 mg/mL). All aliquots were taken after 5 min. Error bars are expressed as 3σ for three independent experiments. The lines are included only as a guide to the eye for better illustration.
Figure 4
Figure 4
Fluorescence intensity at 571 nm of rhodamine B released from S1 suspensions in PBS (pH 7.4)-acetonitrile 95:5 v/v after 5 min of HSA (10 μM) and selected interfering molecules (10 μM) addition. Synthetic urine was dissolved in PBS (pH 7.4)-acetonitrile 95:5 v/v at 10% concentration.
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