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. 2022 May;35(3):779-791.
doi: 10.1007/s13577-021-00655-4. Epub 2022 Feb 14.

MiR-1260b protects against LPS-induced degenerative changes in nucleus pulposus cells through targeting TCF7L2

Shijie Chen  1   2 , Guixia Shi  3 , Jin Zeng  1 , Ping Huang Li  1 , Yi Peng  1 , Zhiyu Ding  1 , Hong Qing Cao  1 , Ruping Zheng  4 , Weiguo Wang  5
Affiliations

MiR-1260b protects against LPS-induced degenerative changes in nucleus pulposus cells through targeting TCF7L2

Shijie Chen et al. Hum Cell. 2022 May.

Abstract

Nucleus pulposus (NP) cells play a critical role in maintaining intervertebral disc integrity through producing the components of extracellular matrix (ECM). NP cell dysfunction, including senescence and hyper-apoptosis, has been regarded as critical events during intervertebral disc degeneration development. In the present study, we found that Transcription Factor 7-Like 2 (TCF7L2) was overexpressed within degenerative intervertebral disc tissue samples, and TCF7L2 silencing improved lipopolysaccharide (LPS)-induced repression on NP cell proliferation, ECM synthesis, and LPS-induced NP cell senescence. miR-1260b directly targeted TCF7L2 and inhibited TCF7L2 expression. miR-1260b overexpression improved LPS-induced degenerative changes in NP cells; more importantly, TCF7L2 overexpression significantly reversed the effects of miR-1260b overexpression on LPS-stimulated degenerative changes within NP cells. For the first time, we demonstrated the function of the miR-1260b/TCF7L2 axis on the phenotypic maintenance of chondrocyte-like NP cells and ECM synthesis by NP cells under LPS stimulation.

Keywords: Intervertebral disc degeneration; Nucleus pulposus cells; Senescence; TCF7L2; miR-1260b.

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