Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Dec;44(6):709-716.
doi: 10.1016/j.bj.2020.05.014. Epub 2020 May 26.

Application of Solidago chilensis and laser improved the repair of burns in diabetic rats

Juliana A R Moreira  1 , Israel C Vasconcelos  1 , José L Fachi  1 , Viviane Theodoro  1 , Rodrigo A Dalia  1 , Andrea A Aro  1 , Edson R Pimentel  2 , Fernanda O G Gaspi  1 , Thiago A M Andrade  1 , Maria E C Amaral  1 , Marcelo A M Esquisatto  1 , Fernanda A S Mendonça  1 , Gláucia M T Santos  3
Affiliations

Application of Solidago chilensis and laser improved the repair of burns in diabetic rats

Juliana A R Moreira et al. Biomed J. 2021 Dec.

Abstract

Background: The repair of burns in diabetic patients is a clinical problem. It is relevant to study alternative therapies that can improve the healing process. Our aim was to investigate the effects of Solidago chilensis associated or not with laser on burns in diabetic rats.

Methods: The animals were divided in four groups (n = 30): C- without treatment; S- S. chilensis extract; L-laser irradiated; LS- laser and S. chilensis. In 7, 14 and 21 days samples were collected after the injury to structural, morphometric and molecular analysis.

Results: Our results demonstrate the association of S. chilensis and laser reduced the inflammatory infiltrate and favored the angiogenesis. In the groups treated only with laser or with the plant extract showed higher levels of VEGF. The low-level laser therapy (LLLT) promoted higher collagen I and reduction of collagen III. It was also observed higher MMP-2 activation and a decreasing of the active isoform of MMP-9 in the S, L and LS groups.

Conclusions: The treatments improved the repair of burns in diabetic rats, since it reduced the inflammatory infiltrate and favored the collagen organization presenting similar effects in the burn repair of the diabetics.

Keywords: Diabetes; InGaP laser; LLLT; Plant extracts; Skin burns; Solidago chilensis.

PubMed Disclaimer

Conflict of interest statement

Conflicts of interest We certify that there is no actual or potential conflicts of interest related to this article exist.

Figures

Fig. 1
Fig. 1
Imunoblotting analysis for Collagen I and III, TGF-β1, VEGF and bFGF in the wound healing process in C, S, L and LS groups in 7 (7 d), 14 (14 d) and 21 (21 d) days after injury. Typical blots are shown above average densitometry results. Values are the mean and standard deviation of each group were compared by ANOVA wich Tukey's post-test (p < 0.05) (∗) significant difference.
Fig. 2
Fig. 2
Zymogram for analyzing the isoforms latent (92 kDa) and active (83 kDa) of MMP-9; and the intermediate isoforms (68 kDa) and active (62 kDa) of MMP-2 in C, S, L and LS groups 7 (7 d), 14 (14 d) and 21 (21 d) days after lesions.
See this image and copyright information in PMC

References

    1. Motley T.A., Gilligan A.M., Lange D.L., Waycaster C.R., Dickerson Jr., J.E. Cost-effectiveness of clostridial collagenase ointment on wound closure in patients with diabetic foot ulcers: economic analysis of results from a multicenter, randomized, open-label trial. J Foot Ankle Res. 2015;8:7. - PMC - PubMed
    1. Zhao J., Li Y.G., Deng K.Q., Yun P., Gong T. Therapeutic effects of static magnetic field on wound healing in diabetic rats. J Diabetes Res. 2017;2017:6305370. - PMC - PubMed
    1. Sandhya S., Sai Kumar P., Vinod K.R., Banji D., Kumar K. Plants as potent anti-diabetic and wound healing agents – a review. Hygeia J Drugs Med. 2011;3:11–19.
    1. Kaya H., Gokdemir M.T., Sogut O., Demir T., Koçarslan S. Effects of folk medicinal plant extract ankaferd blood stopper on burn wound healing. Acta Med Mediterr. 2013;29:497–502.
    1. Goulart S., Moritz M.I., Lang K.L., Liz R., Schenkel E.P., Fröde T.S. Anti-inflammatory evaluation of Solidago chilensis Meyen in a murine model of pleurisy. J Ethnopharmacol. 2007;113:346–353. - PubMed

Publication types