Long non-coding RNA PRNCR1 promotes ovarian cancer cell proliferation, migration and invasion by targeting the miR-653-5p/ELF2 axis

doi:10.1007/s11010-022-04371-x

. 2022 May;477(5):1463-1475.

doi: 10.1007/s11010-022-04371-x. Epub 2022 Feb 15.

Long non-coding RNA PRNCR1 promotes ovarian cancer cell proliferation, migration and invasion by targeting the miR-653-5p/ELF2 axis

Xing Qi¹, Dejun Chen¹, Weichang Yu¹, Liming Wang¹, Lu Liu¹, Xiaoling Tao²

Affiliations

¹ Department of Gynecology, Hubei Provincial Maternal and Child Health Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.745 Wuluo Road, Hongshan District, Wuhan City, Hubei Province, 430070, People's Republic of China.
² Department of Gynecology, Hubei Provincial Maternal and Child Health Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.745 Wuluo Road, Hongshan District, Wuhan City, Hubei Province, 430070, People's Republic of China. xiaolingtaotongji@163.com.

PMID: 35166984
DOI: 10.1007/s11010-022-04371-x

Long non-coding RNA PRNCR1 promotes ovarian cancer cell proliferation, migration and invasion by targeting the miR-653-5p/ELF2 axis

Xing Qi et al. Mol Cell Biochem. 2022 May.

. 2022 May;477(5):1463-1475.

doi: 10.1007/s11010-022-04371-x. Epub 2022 Feb 15.

Authors

Xing Qi¹, Dejun Chen¹, Weichang Yu¹, Liming Wang¹, Lu Liu¹, Xiaoling Tao²

Affiliations

¹ Department of Gynecology, Hubei Provincial Maternal and Child Health Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.745 Wuluo Road, Hongshan District, Wuhan City, Hubei Province, 430070, People's Republic of China.
² Department of Gynecology, Hubei Provincial Maternal and Child Health Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.745 Wuluo Road, Hongshan District, Wuhan City, Hubei Province, 430070, People's Republic of China. xiaolingtaotongji@163.com.

PMID: 35166984
DOI: 10.1007/s11010-022-04371-x

Abstract

Recent studies have shown that prostate cancer-associated long non-coding RNA, PRNCR1, plays crucial roles in the development of multiple human cancers. However, its role in ovarian cancer is barely known. This study was carried out to investigate the role of PRNCR1 and the underlying mechanisms in OC. The expression of PRNCR1 and miR-653-5p in OC cell lines and tissues were detected by qRT-PCR. The expression of ELF2 protein was evaluated by Western blot analysis. Cell proliferation was measured by colony formation and MTT assay. Cell invasion and migration were evaluated by Transwell and wound healing assay. Luciferase reporter assay and RNA-binding protein immunoprecipitation assay were performed to determine the interaction between miR-653-5p and PRNCR1, as well as between miR-653-5p and ELF2. In vivo tumor xenograft model was established to evaluate the role of PRNCR1 in tumor growth. Our results demonstrated that PRNCR1 was significantly upregulated in both OC cell lines and tissues, and high expression of PRNCR1 was correlated with poor survival of OC patients. Overexpression of PRNCR1 accelerated OC cell invasion, migration and proliferation. Besides, the expression of PRNCR1 was negatively correlated with the expression of miR-653-5p, while positively correlated with the expression of E74-like factor 2 in OC tissues. Importantly, ELF2 could target miR-653-5p, and PRNCR1 increased the expression levels of ELF2 by sponging miR-653-5p in OC cells. Furthermore, the miR-145-5p/ELF2 axis was involved in the regulation of PRNCR1 in OC progression in vivo. PRNCR1 promotes OC tumor progress via the miR-653-5p/ELF2 axis and might be a potential therapeutic target for OC.

Keywords: ELF2; Ovarian cancer; PRNCR1; miR-653-5p.

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References

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[1] Center MM, Jemal A, Lortet-Tieulent J, Ward E, Ferlay J, Brawley O, Bray F (2012) International variation in prostate cancer incidence and mortality rates. Eur Urol 61(6):1079–1092. https://doi.org/10.1016/j.eururo.2012.02.054 - DOI - PubMed

[2] Center MM, Jemal A, Lortet-Tieulent J, Ward E, Ferlay J, Brawley O, Bray F (2012) International variation in prostate cancer incidence and mortality rates. Eur Urol 61(6):1079–1092. https://doi.org/10.1016/j.eururo.2012.02.054 - DOI - PubMed

[3] Allemani C, Weir HK, Carreira H, Harewood R, Spika D, Wang XS, Bannon F, Ahn JV, Johnson CJ, Bonaventure A, Marcos-Gragera R, Stiller C, Azevedo e Silva G, Chen WQ, Ogunbiyi OJ, Rachet B, Soeberg MJ, You H, Matsuda T, Bielska-Lasota M, Storm H, Tucker TC, Coleman MP, Group CW (2015) Global surveillance of cancer survival 1995-2009: analysis of individual data for 25,676,887 patients from 279 population-based registries in 67 countries (CONCORD-2). Lancet 385 (9972):977–1010. https://doi.org/10.1016/S0140-6736(14)62038-9

[4] Allemani C, Weir HK, Carreira H, Harewood R, Spika D, Wang XS, Bannon F, Ahn JV, Johnson CJ, Bonaventure A, Marcos-Gragera R, Stiller C, Azevedo e Silva G, Chen WQ, Ogunbiyi OJ, Rachet B, Soeberg MJ, You H, Matsuda T, Bielska-Lasota M, Storm H, Tucker TC, Coleman MP, Group CW (2015) Global surveillance of cancer survival 1995-2009: analysis of individual data for 25,676,887 patients from 279 population-based registries in 67 countries (CONCORD-2). Lancet 385 (9972):977–1010. https://doi.org/10.1016/S0140-6736(14)62038-9

[5] Nakamura K, Nakayama K, Ishibashi T, Ishikawa N, Ishikawa M, Katagiri H, Minamoto T, Sato E, Sanuki K, Yamashita H, Iida K, Sultana R, Kyo S (2016) KRAS/BRAF analysis in ovarian low-grade serous carcinoma having synchronous all pathological precursor regions. Int J Mol Sci. https://doi.org/10.3390/ijms17050625 - DOI - PubMed - PMC

[6] Nakamura K, Nakayama K, Ishibashi T, Ishikawa N, Ishikawa M, Katagiri H, Minamoto T, Sato E, Sanuki K, Yamashita H, Iida K, Sultana R, Kyo S (2016) KRAS/BRAF analysis in ovarian low-grade serous carcinoma having synchronous all pathological precursor regions. Int J Mol Sci. https://doi.org/10.3390/ijms17050625 - DOI - PubMed - PMC

[7] Vang R, Levine DA, Soslow RA, Zaloudek C, Shih Ie M, Kurman RJ (2016) Molecular alterations of TP53 are a defining feature of ovarian high-grade serous carcinoma: a rereview of cases lacking TP53 mutations in the cancer genome atlas ovarian study. Int J Gynecol Pathol 35(1):48–55. https://doi.org/10.1097/PGP.0000000000000207 - DOI - PubMed - PMC

[8] Vang R, Levine DA, Soslow RA, Zaloudek C, Shih Ie M, Kurman RJ (2016) Molecular alterations of TP53 are a defining feature of ovarian high-grade serous carcinoma: a rereview of cases lacking TP53 mutations in the cancer genome atlas ovarian study. Int J Gynecol Pathol 35(1):48–55. https://doi.org/10.1097/PGP.0000000000000207 - DOI - PubMed - PMC

[9] Pheasant M, Mattick JS (2007) Raising the estimate of functional human sequences. Genome Res 17(9):1245–1253. https://doi.org/10.1101/gr.6406307 - DOI - PubMed

[10] Pheasant M, Mattick JS (2007) Raising the estimate of functional human sequences. Genome Res 17(9):1245–1253. https://doi.org/10.1101/gr.6406307 - DOI - PubMed

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Long non-coding RNA PRNCR1 promotes ovarian cancer cell proliferation, migration and invasion by targeting the miR-653-5p/ELF2 axis

Affiliations

Long non-coding RNA PRNCR1 promotes ovarian cancer cell proliferation, migration and invasion by targeting the miR-653-5p/ELF2 axis

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