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. 2022 Sep;46(3):799-810.
doi: 10.1007/s11259-022-09900-z. Epub 2022 Feb 15.

Prevalence, antimicrobial resistance and virulence genes of Salmonella serovars isolated from humans and animals

Probodh Borah  1   2 , Rupam Dutta  1 , Leena Das  1 , Girin Hazarika  1 , Mridusmita Choudhury  2 , Naba Kumar Deka  2 , Dipika Malakar  2 , Md Iftikar Hussain  1 , Luit Moni Barkalita  1
Affiliations

Prevalence, antimicrobial resistance and virulence genes of Salmonella serovars isolated from humans and animals

Probodh Borah et al. Vet Res Commun. 2022 Sep.

Abstract

We investigated the prevalence, antimicrobial susceptibility, antimicrobial resistance and virulence genes of Salmonella isolates recovered from humans and different species of animals. Out of 1231 samples, 88 (7.15%) Salmonella isolates were obtained, among which 21 (23.86%) belonged to Salmonella enterica subsp. enterica sero var. Weltevreden, 22 (25%) to S. Enteritidis, 16 (18.2%) to S. Typhi and 14 (15.9%) to S. Newport; 7 (7.95%) isolates were untypable. Among the 88 isolates, 65.90% showed resistance to gentamicin, 61.36% to tetracycline, 61.18% to cefotaxime, 48.86% to trimethoprim, 45.45% to ampicillin, 11.36% to ceftriaxone, 10.22% to chloramphenicol and 7.95% each to ciprofloxacin and cefepime. Most of the isolates were susceptible, with a low MIC (≤ 0.25 μg/ml) value, to cefepime, cefotaxime, ciprofloxacin, ceftriaxone and co-trimoxazole and with a moderate MIC (0.5-4 μg/ml) to ampicillin, tetracycline, gentamicin and chloramphenicol. The resistance genes blaTEM, tetA and dfrA12 were most prevalent, irrespective of the host of origin of the isolates. While invA was used for molecular detection of Salmonella, other virulence genes, viz. sipA, sipB, sipC, stn and pagN, were also detected in all Salmonella isolates. A total of 38.64% isolates were multidrug-resistant (MDR), and various virulence genes were present among the isolated serovars. This study highlights the importance of continuous monitoring and surveillance for pathogenic Salmonella and their potential risks to both humans and animals.

Keywords: Antimicrobial resistance; Prevalence; Salmonella; Virulence gene.

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