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. 2022 May;36(5):1440-1443.
doi: 10.1038/s41375-022-01517-0. Epub 2022 Feb 15.

Human CD34+ very small embryonic-like stem cells can give rise to endothelial colony-forming cells with a multistep differentiation strategy using UM171 and nicotinamide acid

Alison Domingues  1 , Elisa Rossi  2 , Kamila Bujko  3 , Grégoire Detriche  2   4 , Ulysse Richez  4 , Adeline Blandinieres  2   5 , Magdalena Kucia  1   3 , Janina Ratajczak  1 , David M Smadja  6   7 , Mariusz Z Ratajczak  8   9
Affiliations

Human CD34+ very small embryonic-like stem cells can give rise to endothelial colony-forming cells with a multistep differentiation strategy using UM171 and nicotinamide acid

Alison Domingues et al. Leukemia. 2022 May.

Erratum in

No abstract available

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Conflict of interest statement

University of Louisville filled a patent on ex vivo expansion of VSELs. Inventors (MZR, JR, and MK) do not receive any monetary gratifications from this patent.

Figures

Fig. 1
Fig. 1. Sorted differentiated VSELs into ECs are similar to ECFCs.
A Schematic illustration of the endothelial cell differentiation strategy from VSELs. The different steps over time are summarized and illustrated by phase images of the cells in these different steps along time. First, the VSEL phenotype was confirmed with the small-round-sized cells. After mesodermal induction and endothelial differentiation, cells are bigger and elongated. B Transmission electron microscopy images of VSEL-ECFC cultivated for 24 h on fibrin network on top of pericardial membrane: left panel represents large picture of VSEL-ECFCs on top of fibrin matrices, middle panel represents a higher magnification of the same region than left panel and right panel represent a region with potential Weibel-palade bodies and caveolae. C Longitudinal quantification of migrated VSEL-ECFCs and CB-ECFCs after scratch assay, expressed as a percentage of wound healing (n = 5 per group). Results are expressed as means ± SEM and were analyzed by two-way ANOVA, followed by a Bonferroni post hoc test. D Left panel: representative phase images of formation of pseudo-tube in Matrigel model in vitro after 4 and 8 h from ECFCs in conditioned medium from VSEL-ECFCs and CB-ECFCs. Right panel: quantification of formation of pseudo-tube, expressed as total length of tubes (n = 6 per group). Results are expressed as means ± SEM and were analyzed by two-way ANOVA, followed by a Bonferroni post hoc test. E In vitro and In vivo angiogenic properties of VSEL-ECFCs. Representative fluorescence images of the 3D sprouting assay. Quantification of the sprouting, expressed as total network per bead and, number of sprouts per bead and average sprout length (n = 6 per group). Results are expressed as means ± SEM and were analyzed using Wilcoxon test. *p ≤ 0.05.
See this image and copyright information in PMC

References

    1. Smadja DM, Melero Martin JM, Eikenboom J, Bowman M, Sabatier F, Randi AM. Standardization of methods to quantify and culture endothelial colony-forming cells derived from peripheral blood: position paper from the International Society on Thrombosis and Haemostasis SSC. J Thromb Haemost. 2019;17:1190–4. doi: 10.1111/jth.14462. - DOI - PMC - PubMed
    1. Ratajczak MZ, Ratajczak J, Suszynska M, Miller DM, Kucia M, Shin D-M. A novel view of the adult stem cell compartment from the perspective of a quiescent population of very small embryonic-like stem cells. Circ Res. 2017;120:166–78. doi: 10.1161/CIRCRESAHA.116.309362. - DOI - PMC - PubMed
    1. Guerin CL, Loyer X, Vilar J, Cras A, Mirault T, Gaussem P, et al. Bone-marrow-derived very small embryonic-like stem cells in patients with critical leg ischaemia: evidence of vasculogenic potential. Thromb Haemost. 2015;113:1084–94. doi: 10.1160/TH14-09-0748. - DOI - PubMed
    1. Havens AM, Sun H, Shiozawa Y, Jung Y, Wang J, Mishra A, et al. Human and murine very small embryonic-like cells represent multipotent tissue progenitors, in vitro and in vivo. Stem cells Dev. 2014;23:689–701. doi: 10.1089/scd.2013.0362. - DOI - PMC - PubMed
    1. Kennedy CC, Brown EE, Abutaleb NO, Truskey GA. Development and application of endothelial cells derived from pluripotent stem cells in microphysiological systems models. Front Cardiovasc Med. 2021;8:625016. doi: 10.3389/fcvm.2021.625016. - DOI - PMC - PubMed

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