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. 2021;29(7-8):1616-1620.
doi: 10.1080/09273948.2020.1758731. Epub 2020 May 28.

S100 proteins, cytokines, and chemokines as tear biomarkers in children with juvenile idiopathic arthritis-associated uveitis

Sheila T Angeles-Han  1 , Virginia Miraldi Utz  2 , Sherry Thornton  1 , Grant Schulert  1 , Jackeline Rodriguez-Smith  1 , Adam Kauffman  2   3 , Alyssa Sproles  1 , Najima Mwase  1 , Theresa Hennard  1 , Alexei Grom  1 , Mekibib Altaye  4 , Gary N Holland  5
Affiliations

S100 proteins, cytokines, and chemokines as tear biomarkers in children with juvenile idiopathic arthritis-associated uveitis

Sheila T Angeles-Han et al. Ocul Immunol Inflamm. 2021.

Abstract

Purpose: Biomarkers for juvenile idiopathic arthritis-associated uveitis (JIA-U) are needed. We aimed to measure inflammatory biomarkers in tears as a non-invasive method to identify biomarkers of uveitis activity.

Methods: Tears were collected from children with JIA-U (n=20) and pediatric controls (n=20) using Schirmer strips. S100A8, A9, A12, IL-18, IL-8, IP-10, MCP-1, RANTES, and sICAM-1 were measured by ELISA and Luminex assays. Levels of biomarkers were compared between children with JIA-U and controls, and active and inactive JIA-U.

Results: IL-8, sICAM-1, and S100A12 levels were similar between JIA-U and controls, but differed by activity. Active JIA-U had significantly increased S100A12 compared to inactive JIA-U (mean 27,722.5 pg/ML (SE 1.3) vs. 5,937.2 (1.3), p=0.002), IL-8 (73.5 [1.2] vs. 36.2 [1.2], p=0.009), and sICAM-1 (15,822.7 [1.2) vs. 8,778.0 [1.6], p=0.024).

Conclusion: We detected inflammatory biomarkers non-invasively in tears of children with JIA-U. IL-8, sICAM-1, and S100A12 are potential biomarkers for uveitis activity.

Keywords: biomarkers; juvenile idiopathic arthritis; uveitis.

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Conflict of interest statement

Declaration of Interest: Dr. Virginia Utz is an investigator for Retrophin (R) and receives royalties from Springer for a textbook, both not related to this study.

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