Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 May 22;89(2):233-8.
doi: 10.1016/0022-1759(86)90363-7.

Use of particulate antigen to enhance primary in vitro antibody responses to trinitrophenyl-keyhole limpet hemocyanin

T M Buttke, S Pourbohloul, N W Miller

Use of particulate antigen to enhance primary in vitro antibody responses to trinitrophenyl-keyhole limpet hemocyanin

T M Buttke et al. J Immunol Methods. .

Abstract

Methods are described for the efficient induction of primary anti-hapten antibody responses to trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH) in vitro. Mouse leukocytes stimulated in vitro with particulate (bentonite-adsorbed) TNP-KLH demonstrated 10-fold more IgM-secreting, plaque-forming cells (PFC) as measured by a hemolytic plaque assay than did leukocytes stimulated with soluble TNP-KLH. The T dependency of the enhanced antibody response was confirmed using highly enriched B and T lymphocyte populations. These methods should greatly facilitate studies regarding primary in vitro antibody responsiveness to T-dependent antigens.

PubMed Disclaimer

Publication types

LinkOut - more resources