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. 2022 Oct;36(10):7312-7324.
doi: 10.1007/s00464-022-09122-z. Epub 2022 Feb 19.

Laparoscopic sleeve gastrectomy for morbid obesity improves gut microbiota balance, increases colonic mucosal-associated invariant T cells and decreases circulating regulatory T cells

Naoki Fukuda  1 , Toshiyasu Ojima  2 , Keiji Hayata  1 , Masahiro Katsuda  1 , Junya Kitadani  1 , Akihiro Takeuchi  1 , Taro Goda  1 , Yoko Ueda  3 , Hiroshi Iwakura  3 , Masahiro Nishi  3 , Hiroki Yamaue  1
Affiliations

Laparoscopic sleeve gastrectomy for morbid obesity improves gut microbiota balance, increases colonic mucosal-associated invariant T cells and decreases circulating regulatory T cells

Naoki Fukuda et al. Surg Endosc. 2022 Oct.

Abstract

Background: Laparoscopic sleeve gastrectomy (LSG) for morbid obesity may improve gut microbiota balance and decrease chronic inflammation. This study examines the changes in gut microbiota and immune environment, including mucosal-associated invariant T cells (MAIT cells) and regulatory T cells (Treg cells) caused by LSG.

Methods: Ten morbidly obese patients underwent LSG at our institution between December 2018 and March 2020. Flow cytometry for Th1/Th2/Th17 cells, Treg cells and MAIT cells in peripheral blood and colonic mucosa and 16S rRNA analysis of gut microbiota were performed preoperatively and then 12 months postoperatively.

Results: Twelve months after LSG, the median percent total weight loss was 30.3% and the median percent excess weight loss was 66.9%. According to laboratory data, adiponectin increased, leptin decreased, and chronic inflammation improved after LSG. In the gut microbiota, Bacteroidetes and Fusobacteria increased after LSG, and indices of alpha diversity increased after LSG. In colonic mucosa, the frequency of MAIT cells increased after LSG. In peripheral blood, the frequency of Th1 cells and effector Treg cells decreased after LSG.

Conclusions: After LSG for morbid obesity, improvement in chronic inflammation in obesity is suggested by change in the constituent bacterial species, increase in the diversity of gut microbiota, increase in MAIT cells in the colonic mucosa, and decrease in effector Treg cells in the peripheral blood.

Keywords: Bariatric surgery; Gut microbiota; Laparoscopic sleeve gastrectomy; MAIT cell; Metabolic surgery; Treg cell.

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