Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar 15;145(11):868-870.
doi: 10.1161/CIRCULATIONAHA.121.057613. Epub 2022 Feb 21.

e-Cigarette Aerosol Reduces Left Ventricular Function in Adolescent Mice

Evan W Neczypor  1 , Ty A Saldaña  1 , Matthew J Mears  1 , David M Aslaner  1 , Yael-Natalie H Escobar  1 , Matthew W Gorr  1 , Loren E Wold  1
Affiliations

e-Cigarette Aerosol Reduces Left Ventricular Function in Adolescent Mice

Evan W Neczypor et al. Circulation. .
No abstract available

Keywords: adolescent; e-cigarette; heart function; vaping.

PubMed Disclaimer

Figures

Figure
Figure
Male and female mice were exposed to either filtered air (FA), electronic cigarette aerosol without nicotine (EC), or with nicotine (EC(+Nic)). Data are expressed as mean ± SEM. *: p<0.05 via unpaired Student’s t-test, or one- or two-way ANOVA with post-hoc Tukey’s multiple comparison test. Statistical tests for RT-qPCR data were conducted on ΔCT values. Panel A: Heart weight/tibia length ratio of male and female mice (n = 8–19) after 3 months exposure. Panel B: Echocardiographic analysis of the left ventricle (LV) of male (n = 24–30) and female (n = 14–22) mice after 3 months of exposure. Male EC(+Nic) mice had reduced fractional shortening (%FS) and reduced transmitral early (E) to late (A) ventricular filling peak velocity ratio (E/A ratio) (*=P<0.05 via ANOVA). Panel C: PV loops from male mice exposed for 3 months: slope of the end-systolic pressure-volume relationship curve is lowered in EC and EC(+Nic) mice as indicated by reduced Ees, preload recruitable stroke work is also reduced in EC(+Nic) mice compared to FA, and Tau is unchanged (*=P<0.05 via ANOVA) Panel D: Representative perivascular images from male mice exposed for 3 months. Sections were subject to Masson’s trichrome staining and imaged using a bright-field light microscope at 60x magnification (top). Percent fibrosis was quantified as percent area of blue staining region averaged across at least 3 images per mouse (n = 5) using ImageJ software (bottom left). ELISA targeting type I collagen in the LV of male mice exposed for 3 months (n = 5, *=P<0.05, via ANOVA) (bottom right). Panel E: Gene expression of COL1A1 and COL3A1 in the LV of male mice exposed for either 3 weeks (3wk, top) or 3 months (3mo, bottom) (n = 4, *=P<0.05 via ANOVA). Panel F: Volcano plots from deseq2 analysis of RNA-sequencing of LV from hearts of adolescent and adult male mice; each dot represents a gene-blue or red color indicates the gene was down-regulated or up-regulated, respectively, with a cutoff value of 2-fold change and adjusted P<0.05. Panel G: Concentration of biomarkers in the serum of mice exposed to FA, EC, and EC(+Nic) (n = 3–6) for 3 months collected via cardiac puncture within 24 hours of exposure (*=P<0.05 via ANOVA). Panel H: Concentration of nicotine and metabolites in serum of male (blue) and female (red) EC(+Nic) mice exposed for 3 months (n = 10) collected via submandibular bleed within 4 hours of exposure (top) (*=P<0.05 via ANOVA).
See this image and copyright information in PMC

References

    1. Miech R, Johnston L, O’Malley PM, Bachman JG, Patrick ME. Trends in Adolescent Vaping, 2017–2019. N Engl J Med. 2019;381:1490–1491. doi: 10.1056/NEJMc1910739. - DOI - PMC - PubMed
    1. Chun LF, Moazed F, Calfee CS, Matthay MA, Gotts JE. Pulmonary toxicity of e-cigarettes. Am J Physiol Lung Cell Mol Physiol. 2017;313:L193–L206. doi: 10.1152/ajplung.00428.2017. - DOI - PMC - PubMed
    1. Nelin TD, Joseph AM, Gorr MW, Wold LE. Direct and indirect effects of particulate matter on the cardiovascular system. Toxicol Lett. 2012;208:293–299. doi: 10.1016/j.toxlet.2011.11.008. - DOI - PMC - PubMed
    1. Tanner J-A, Novalen M, Jatlow P, Huestis MA, Murphy SE, Kaprio J, Kankaanpää A, Galanti L, Stefan C, George TP, et al. Nicotine metabolite ratio (3-hydroxycotinine/cotinine) in plasma and urine by different analytical methods and laboratories: implications for clinical implementation. Cancer Epidemiol Biomarkers Prev. 2015;24:1239–1246. doi: 10.1158/1055-9965.EPI-14-1381. - DOI - PMC - PubMed
    1. Lee YO, Morgan-Lopez AA, Nonnemaker JM, Pepper JK, Hensel EC, Robinson RJ. Latent Class Analysis of E-cigarette Use Sessions in Their Natural Environments. Nicotine Tob Res. 2019;21:1408–1413. doi: 10.1093/ntr/nty164. - DOI - PubMed