Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Feb 21;204(3):186.
doi: 10.1007/s00203-022-02793-1.

Detection of capsular genotypes of methicillin-resistant Staphylococcus aureus and clonal distribution of the cap5 and cap8 genes in clinical isolates

Gabriela Echániz-Aviles  1 , Maria Elena Velazquez-Meza  2 , Brian Rodríguez-Arvizu  1 , Maria Noemi Carnalla-Barajas  1 , Araceli Soto Noguerón  1
Affiliations

Detection of capsular genotypes of methicillin-resistant Staphylococcus aureus and clonal distribution of the cap5 and cap8 genes in clinical isolates

Gabriela Echániz-Aviles et al. Arch Microbiol. .

Abstract

Up until now, the capsular polysaccharides of Staphylococcus aureus have been classified into 11 types, of which only 2 types 5 and 8; (encoded by the genes cap5 and cap8, respectively) are present in 80-90% of clinically significant strains. The aim of the present study was to detect the capsular genotypes of methicillin-resistant S. aureus (MRSA) clinical isolates and determined their clonal distribution. A total of 262 MRSA clinical isolates from different hospitals in Mexico were analyzed by PCR to determine the genetic characteristics of their capsule expression. Pulsed-field gel electrophoresis and multilocus sequence typing were used to characterize the isolates. The analysis of the capsular genotypes among MRSA isolates showed that 245 isolates (93.5%) contained the cap5 gene, and that the remaining 17 (6.5%) encoded the cap8 gene. The MRSA isolates were grouped into four clonal groups. The identification of the capsular genotypes of clinical isolates of MRSA is important information because potential vaccine formulations against S. aureus involve capsular polysaccharides.

Keywords: Capsular genotypes; Clinical isolates; MRSA; cp5; cp8.

PubMed Disclaimer

Conflict of interest statement

None declared.

Figures

Fig. 1
Fig. 1
Distribution of capsule genotypes (cap5 and cap8) among MRSA clinical isolates
Fig. 2
Fig. 2
Examples of pulsed field gel electrophoresis profiles obtained for MRSA clones of clinical isolates and international clones. Lanes 1 and 12 lambda ladder used a molecular size (MW) markers, 2–4 representatives clinical isolates of clones EMRSA-16, lines 5–7 New York-Japan, Iberian and USA300 clones, respectively. Lanes 8–11 control strains EMRSA16, BK2464, HPV107 and USA300
Fig. 3
Fig. 3
Analysis of eBURST of the ST5 and ST8 clonal complex of MRSA. The relatedness between isolates in the MRSA MLST database that shared alleles at seven loci with the allelic profile of ST5 and ST8 is displayed as a dendrogram
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Aires de Sousa M, de Lencastre H. Bridges from hospitals to the laboratory: genetic portraits of methicillin-resistant Staphylococcus aureus clones. FEMS Immunol Med Microbiol. 2004;40(2):101–111. doi: 10.1016/S0928-8244(03)00370-5. - DOI - PubMed
    1. Aires de Sousa M, Bartzavali C, Spiliopoulou I, Santos I, Crisostomo I, de Lencastre H. Two international methicillin-resistant Staphylococcus aureus clones endemic in a university hospital in Patras, Greece. J Clin Microbiol. 2003;41(5):2027–2032. doi: 10.1128/JCM.41.5.2027-2032.2003. - DOI - PMC - PubMed
    1. Challagundla L, Reyes J, Rafiqullah I, Sordelli DO, Echaniz-Aviles G, Velazquez-Meza ME, Castillo-Ramírez S, Fittipaldi N, Feldgarden M, Chapman SB, Calderwood MS, Carvajal LP, Rincon S, Hanson B, Planet PJ, Arias CA, Diaz L, Robinson DA. Phylogenomic classification and the evolution of clonal complex 5 methicillin-resistant Staphylococcus aureus in the Western Hemisphere. Front Microbiol. 2018;9:1–14. doi: 10.3389/fmicb.2018.01901. - DOI - PMC - PubMed
    1. Chung M, de Lencastre H, Matthews P, Tomasz A, Adamsson I, Aires de Sousa M, Camou T, Cocuzza C, Corso A, Couto I, Dominguez A, Gniadkowski M, Goering R, Gomes A, Kikuchi K, Marchese A, Mato R, Melter O, Oliveira D, Palacio R, Sá-Leão R, Santos Sanches I, Song JH, Tassios PT, Villari P, Multilaboratory Project Collaborators Molecular typing of methicillin- resistant Staphylococcus aureus by pulsed-field gel electrophoresis: comparison of results obtained in a multilaboratory effort using identical protocols and MRSA strains. Microb Drug Resist. 2000;6(3):189–198. doi: 10.1089/mdr.2000.6.189. - DOI - PubMed
    1. Chung M, Dickinson G, de Lencastre H, Tomasz A. International clones of methicillin-resistant Staphylococcus aureus in two hospitals in Miami, Florida. J Clin Microbiol. 2004;42(2):542–547. doi: 10.1128/JCM.42.2.542-547.2004. - DOI - PMC - PubMed

MeSH terms