Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Feb;29(2):1197-1209.
doi: 10.1016/j.sjbs.2021.09.046. Epub 2021 Sep 17.

Antioxidant and antimicrobial activities of Spirulina platensis extracts and biogenic selenium nanoparticles against selected pathogenic bacteria and fungi

Abdel-Moneim Eid Abdel-Moneim  1 , Mohamed T El-Saadony  2 , Abdelrazeq M Shehata  3 , Ahmed M Saad  4 , Sami Ali Aldhumri  5 , Sahar M Ouda  5   6 , Noura M Mesalam  1
Affiliations

Antioxidant and antimicrobial activities of Spirulina platensis extracts and biogenic selenium nanoparticles against selected pathogenic bacteria and fungi

Abdel-Moneim Eid Abdel-Moneim et al. Saudi J Biol Sci. 2022 Feb.

Abstract

This study investigated the antimicrobial and antioxidant activity of three Spirulina extracts (methanol, acetone, and hexane) and the biological selenium nanoparticles (SeNPs) fabricated by Bacillus subtilis AL43. The results showed that Spirulina extracts exhibited antimicrobial activity against tested pathogens. Besides, Spirulina extracts significantly scavenged ABTS and DPPH radicals in a dose-dependent manner. The methanolic extract had higher total phenolic content, antimicrobial activity, and antioxidant activity than other extracts. The selenium nanoparticles were synthesized by Bacillus subtilis AL43 under aerobic conditions and were characterized as spherical, crystalline with a size of 65.23 nm and a net negative charge of -22.7. We evidenced that SeNPs possess considerable antimicrobial activity against three gram-positive, three gram-negative bacteria, and three strains from both Candida sp. and Aspergillus sp. Moreover, SeNPs were able to scavenge ABTS and DPPH radicals in a dose-dependent manner. An association was found between the total phenolic content of Spirulina and SeNPs and their biological activities. Our results indicate that Spirulina and SeNPs with significant antimicrobial and antioxidant activities seem to be successful candidates for safe and reliable medical applications.

Keywords: Antimicrobial; Antioxidant; Bacillus subtilis; Biogenic SeNPs; Green nanotechnology; Spirulina platensis.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
UV–Vis spectra of SeNPs synthesized by B. subtilis AL43.
Fig. 2
Fig. 2
TEM image of biogenic selenium nanoparticles (SeNPs) synthesized by Bacillus subtilis AL43.
Fig. 3
Fig. 3
Size distribution (A) and zeta potential (B) of SeNPs synthesized by B. subtilis AL43.
Fig. 4
Fig. 4
FT-IR spectrum of SeNPs synthesized by B. subtilis AL43.
Fig. 5
Fig. 5
MIC levels of Spirulina extracts (methanol, hexane, and acetone) against (A) tested bacteria, and (B) tested fungi, data are presented as mean ± SE.
Fig. 6
Fig. 6
MIC levels of biogenic selenium nanoparticles (SeNPs) against (A) tested bacteria, and (B) tested fungi, data are presented as mean ± SE.
Fig. 7
Fig. 7
Scavenging activity of (A) Spirulina extracts and (B) biogenic selenium nanoparticles (SeNPs, μg/mL) against ABTS• and DPPH• radicals at room temperature, data are presented as mean ± SE.
See this image and copyright information in PMC

References

    1. Abbas H.S., Abou Baker D.H., Ahmed E.A. Cytotoxicity and antimicrobial efficiency of selenium nanoparticles biosynthesized by Spirulina platensis. Arch. Microbiol. 2021;203:523–532. - PubMed
    1. Abd El-Hack M.E., Abdelnour S.A., Abd El-Moneim A.E., Arif M., Khafaga A., Shaheen H., Samak D., Swelum A.A. Putative impacts of phytogenic additives to ameliorate lead toxicity in animal feed. Environ. Sci. Pollut. Res. 2019;26:23209–23218. - PubMed
    1. Abd El-Hack M.E., Alagawany M., Abdel-Moneim A.-M.-E., Mohammed N.G., Khafaga A.F., Bin-Jumah M., Othman S.I., Allam A.A., Elnesr S.S. Cinnamon (Cinnamomum zeylanicum) oil as a potential alternative to antibiotics in poultry. Antibiotics. 2020;9:210–221. - PMC - PubMed
    1. Abd El-Hack M.E., El-Saadony M.T., Shafi M.E., Alshahrani O.A., Saghir S.A., Al-Wajeeh A.S., Al-Shargi O.Y., Taha A.E., Mesalam N.M., Abdel-Moneim A.-M.-E. Prebiotics can restrict Salmonella populations in poultry: a review. Anim. Biotechnol. 2021;19:1–10. - PubMed
    1. Abd El-Hack M.E., El-Saadony M.T., Shafi M.E., Zabermawi N.M., Arif M., Batiha G.E., Khafaga A.F., Abd El-Hakim Y.M., Al-Sagheer A.A. Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications: a review. Int. J. Biol. Macromol. 2020;1(164):2726–2744. - PubMed

LinkOut - more resources