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Review
. 2022 Feb 7:13:834180.
doi: 10.3389/fphys.2022.834180. eCollection 2022.

Spotlight on the Binding Affinity of Ion Channels for Phosphoinositides: From the Study of Sperm Flagellum

Takafumi Kawai  1 , Yasushi Okamura  1   2
Affiliations
Review

Spotlight on the Binding Affinity of Ion Channels for Phosphoinositides: From the Study of Sperm Flagellum

Takafumi Kawai et al. Front Physiol. .

Abstract

The previous studies revealed that many types of ion channels have sensitivity to PtdIns(4,5)P2, which has been mainly shown using heterologous expression system. On the other hand, there remains few evidence showing that PtdIns(4,5)P2 natively regulate the ion channel activities in physiological context. Our group recently discovered that a sperm specific K+ channel, Slo3, is natively regulated by PtdIns(4,5)P2 in sperm flagellum. Very interestingly, a principal piece, to which Slo3 specifically localized, had extremely low density of PtdIns(4,5)P2 compared to the regular cell plasma membrane. Furthermore, our studies and the previous ones also revealed that Slo3 had much stronger PtdIns(4,5)P2 affinity than KCNQ2/3 channels, which are widely regulated by endogenous PtdIns(4,5)P2 in neurons. Thus, the high-PtdIns(4,5)P2 affinity of Slo3 is well-adapted to the specialized PtdIns(4,5)P2 environment in the principal piece. This study sheds light on the relationship between PtdIns(4,5)P2-affinity of ion channels and their PtdIns(4,5)P2 environment in native cells. We discuss the current understanding about PtdIns(4,5)P2 affinity of diverse ion channels and their possible regulatory mechanism in native cellular environment.

Keywords: KCNQ; Slo3; ion channel; phosphoinositides; sperm flagellum; voltage-sensing phosphatase.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Possible relationship between PtdIns(4,5)P2 concentration and its affinity for ion channels. (A) Voltage-sensing phosphatase (VSP) reduces the PtdIns(4,5)P2 levels in sperm flagellum, regulating Slo3 activity. The figure is illustrated based on the finding in our previous report (Kawai et al., 2019). The PtdIns(4,5)P2 is detected by freeze-fracture electron microscopy. The VSP changes the distribution of PtdIns(4,5)P2 levels and regulates Slo3 activity. The Slo3 activity was measured by perforated patch clamp recording (Kawai et al., 2019). (B) Diagram showing the different PtdIns(4,5)P2 dependency among different ion channels.
See this image and copyright information in PMC

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