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. 2022 Aug;22(6):2349-2362.
doi: 10.1111/1755-0998.13603. Epub 2022 Mar 8.

DNA barcodes highlight genetic diversity patterns in rodents from lowland desert and Andean areas in Argentina

Agustina Alejandra Ojeda  1 , Agustina Novillo  2 , Cecilia Lanzone  3 , María Daniela Rodríguez  4 , Maria Fernanda Cuevas  1 , Jorge Pablo Jayat  5 , Pablo Teta  6 , Ricardo Alberto Ojeda  1 , Alex Borisenko  7
Affiliations

DNA barcodes highlight genetic diversity patterns in rodents from lowland desert and Andean areas in Argentina

Agustina Alejandra Ojeda et al. Mol Ecol Resour. 2022 Aug.

Abstract

Rodents are an important component of South America fauna. Their high diversity has motivated researchers to continually review their taxonomy, genetic diversity, species limits, and phylogenetic relationships. Here, we applied DNA-barcodes for assessing the taxonomic and genetic diversity in the two major lineages of South American rodents: caviomorphs and sigmodontines. We analysed 335 COI barcodes in 34 morphologically determined species from 39 localities along central Andes and arid lands of Argentina. Neighbour-joining and maximum likelihood reconstruction provided clear separation between species. The Barcode Index number and Bayesian Poisson tree processes were used to confirm concordance between sequence clusters and species designations by taxonomy. We found deep divergence within the Phyllotis xanthopygus species complex, with distances up to 13.0% between geographically separated lineages. Minor divergences (3.30% and 2.52%) were found within Abrothrix hirta, and Tympanoctomys barrerae, respectively, with differentiation in their genetic lineages. Also, we documented geographically separated clusters for Akodon spegazzinii and A. oenos with up to 2.3% divergence, but clustering methods failed to distinguish them as different species. Sequence results show a clear barcode gap with a mean intraspecific divergence (0.56%) versus a minimum nearest-neighbour distance averaging (10.1%). Distances between congeneric species varied from 4.1 to 14%, with the exception of two related forms within Euneomys and the sister species Akodon spegazzinii and A. oenos. This study constitutes a substantial contribution to the global barcode reference library. It provides insights into the complex phylogeographic patterns and speciation scenarios in rodents, while highlighting areas that require in-depth taxonomic and integrative research.

Keywords: COI; Caviomorpha; DNA barcoding; Sigmodontinae; South America; species identification.

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References

REFERENCES

    1. Akaike, H. (1974). A new look at the statistical model identification. IEEE Transactions on Biomedical Engineering, 19(6), 716-723.
    1. Albright, J. C. (2004). Phylogeography of the sigmodontine rodent, Phyllotis xanthopygus, and a test of the sensitivity of nested clade analysis to elevation-based alternative distances (p. 46). Ph.D. Dissertation. The Florida State University.
    1. Andrade, L. D., Martínez, J. J., Barquez, R., Martín, E., & Ferro, I. (2021). Código de barras de ADN para la delimitación de especies en roedores sigmodontinos (Cricetidae) del norte argentino, con especial énfasis en aquellas de las Yungas. Mastozoologia Neotroical, 28(1), e0541. https://doi.org/10.31687/saremMN.21.28.1.0.22
    1. Antoine, P., Marivaux, L., Croft, D. A., Billet, G., Ganerød, M., Jaramillo, C., & Gismondi, R. S. (2011). Middle Eocene rodents from Peruvian Amazonia reveal the pattern and timing of caviomorph origins and biogeography. Proceedings of the Royal Society B: Biological Sciences, 279, 1319-1326.
    1. Borisenko, A. V., Lim, B. K., Ivanova, N. V., Hanner, R. H., & Hebert, P. D. N. (2008). DNA barcoding in surveys of small mammal communities: A field study in Suriname. Molecular Ecology Resource, 8, 471-479.

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